Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury

Mitsuru Hanada, Yuki Sugiura, Ryuichi Shinjo, Noritaka Masaki, Shiro Imagama, Naoki Ishiguro, Yukihiro Matsuyama, Mitsutoshi Setou

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We determined quantitative and qualitative alterations in lipids during the occurrence and progression of spinal cord injury (SCI) in rats to identify potential clinical indicators of SCI pathology. Imaging mass spectrometry (IMS) was used to visualize twelve molecular species of phosphatidylcholine (PC) on thin slices of spinal cord with SCI. In addition, twelve species of phospholipids and five species of prostaglandins (PGs) were quantified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) of lipid extracts from control/injured spinal cords. Unique distribution patternswere observed for phospholipids with different fatty acid compositions, and distinct dynamic changes were seen in both their amounts and their distributions in tissue as tissue damage resulting from SCI progressed. In particular, PCs containing docosahexaenoic acid localized to the large nucleus in the anterior horn region at one day post-SCI and rapidly decreased thereafter. In contrast, PCs containing arachidonic acid (AA-PCs) were normally found in the posterior horn region and were intensely and temporarily elevated one week after SCI. Lysophosphatidylcholines (LPCs) also increased at the same SCI stage and in regions with elevated AA-PCs, indicating the release of AA and the production of PGs. Moreover, LC-ESI-MS/MS analysis of lipid extracts from the spinal cord tissue at the impact site demonstrated a peak in PGE2 that reflected the elevation/reduction pattern of AA-PCs and LPC. Although further investigation is required, we suggest that invasive immune cells that penetrated from the impaired blood-brain barrier at 1-2 weeks post-SCI may have produced LPCs, released AA from AA-PCs, and produced PGs in SCI tissue at sites enriched in AA-PCs/LPC.

Original languageEnglish
Pages (from-to)1873-1884
Number of pages12
JournalAnalytical and Bioanalytical Chemistry
Volume403
Issue number7
DOIs
Publication statusPublished - 2012 Jun
Externally publishedYes

Fingerprint

Spinal Cord Injuries
Prostaglandins
Rats
Phospholipids
Lysophosphatidylcholines
Spinal Cord
Tissue
Horns
Lipids
Mass spectrometry
Electrospray Ionization Mass Spectrometry
Docosahexaenoic Acids
Tissue Distribution
Tandem Mass Spectrometry
Blood-Brain Barrier
Phosphatidylcholines
Dinoprostone
Arachidonic Acid
Liquid Chromatography
Electrospray ionization

Keywords

  • Imaging mass spectrometry
  • LC-ESI-MS/MS
  • Phospholipids
  • Prostaglandins
  • Spinal cord injury

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury. / Hanada, Mitsuru; Sugiura, Yuki; Shinjo, Ryuichi; Masaki, Noritaka; Imagama, Shiro; Ishiguro, Naoki; Matsuyama, Yukihiro; Setou, Mitsutoshi.

In: Analytical and Bioanalytical Chemistry, Vol. 403, No. 7, 06.2012, p. 1873-1884.

Research output: Contribution to journalArticle

Hanada, M, Sugiura, Y, Shinjo, R, Masaki, N, Imagama, S, Ishiguro, N, Matsuyama, Y & Setou, M 2012, 'Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury', Analytical and Bioanalytical Chemistry, vol. 403, no. 7, pp. 1873-1884. https://doi.org/10.1007/s00216-012-5900-3
Hanada, Mitsuru ; Sugiura, Yuki ; Shinjo, Ryuichi ; Masaki, Noritaka ; Imagama, Shiro ; Ishiguro, Naoki ; Matsuyama, Yukihiro ; Setou, Mitsutoshi. / Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury. In: Analytical and Bioanalytical Chemistry. 2012 ; Vol. 403, No. 7. pp. 1873-1884.
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AU - Hanada, Mitsuru

AU - Sugiura, Yuki

AU - Shinjo, Ryuichi

AU - Masaki, Noritaka

AU - Imagama, Shiro

AU - Ishiguro, Naoki

AU - Matsuyama, Yukihiro

AU - Setou, Mitsutoshi

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AB - We determined quantitative and qualitative alterations in lipids during the occurrence and progression of spinal cord injury (SCI) in rats to identify potential clinical indicators of SCI pathology. Imaging mass spectrometry (IMS) was used to visualize twelve molecular species of phosphatidylcholine (PC) on thin slices of spinal cord with SCI. In addition, twelve species of phospholipids and five species of prostaglandins (PGs) were quantified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) of lipid extracts from control/injured spinal cords. Unique distribution patternswere observed for phospholipids with different fatty acid compositions, and distinct dynamic changes were seen in both their amounts and their distributions in tissue as tissue damage resulting from SCI progressed. In particular, PCs containing docosahexaenoic acid localized to the large nucleus in the anterior horn region at one day post-SCI and rapidly decreased thereafter. In contrast, PCs containing arachidonic acid (AA-PCs) were normally found in the posterior horn region and were intensely and temporarily elevated one week after SCI. Lysophosphatidylcholines (LPCs) also increased at the same SCI stage and in regions with elevated AA-PCs, indicating the release of AA and the production of PGs. Moreover, LC-ESI-MS/MS analysis of lipid extracts from the spinal cord tissue at the impact site demonstrated a peak in PGE2 that reflected the elevation/reduction pattern of AA-PCs and LPC. Although further investigation is required, we suggest that invasive immune cells that penetrated from the impaired blood-brain barrier at 1-2 weeks post-SCI may have produced LPCs, released AA from AA-PCs, and produced PGs in SCI tissue at sites enriched in AA-PCs/LPC.

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KW - Prostaglandins

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