Spatiotemporal expression of TRPM4 in the mouse cochlea

Mayumi Sakuraba, Junko Murata, Ryoichi Teruyama, Kazusaku Kamiya, Junji Yamaguchi, Hideyuki Okano, Yasuo Uchiyama, Katsuhisa Ikeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study was conducted to elucidate the presence of the transient receptor potential cation channel subfamily M member 4, TRPM4, in the mouse inner ear. TRPM4 immunoreactivity (IR) was found in the cell body of inner hair cells (IHCs) in the organ of Corti in the apical side of marginal cells of the stria vascularis, in the apical portion of the dark cells of the vestibule, and in a subset of the type II neurons in the spiral ganglion. Subsequently, changes in the distribution and expression of TRPM4 in the inner ear during embryonic and postnatal developments were also evaluated. Immunohistochemical localization demonstrated that the emergence of the TRPM4-IR in IHCs occurs shortly before the onset of hearing, whereas that in the marginal cells happens earlier, at the time of birth, coinciding with the onset of endolymph formation. Furthermore, semiquantitative real-time PCR assay showed that expressions of TRPM4 in the organ of Corti and in the stria vascularis increased dramatically at the onset of hearing. Because TRPM4 is a Ca2+-activated monovalent-selective cation channel, these findings imply that TRPM4 contributes to potassium ion transport, essential for the signal transduction in IHCs and the formation of endolymph by marginal cells.

Original languageEnglish
Pages (from-to)1409-1418
Number of pages10
JournalJournal of neuroscience research
Volume92
Issue number10
DOIs
Publication statusPublished - 2014 Oct

Keywords

  • Endolymph
  • Inner hair cell
  • Marginal cell
  • Stria vascularis
  • TRP channel

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Spatiotemporal expression of TRPM4 in the mouse cochlea'. Together they form a unique fingerprint.

  • Cite this

    Sakuraba, M., Murata, J., Teruyama, R., Kamiya, K., Yamaguchi, J., Okano, H., Uchiyama, Y., & Ikeda, K. (2014). Spatiotemporal expression of TRPM4 in the mouse cochlea. Journal of neuroscience research, 92(10), 1409-1418. https://doi.org/10.1002/jnr.23410