Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice

Ryo Yamada, Hirohisa Horinouch, Kosuke Tsukada

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Tumor hypoxia is considered a potential therapeutic problem because it reduces the effects of radiation therapy. Clinical experience has shown that long-term tumor oxygenation cannot be achieved with oxygen inhalation, but the mechanisms behind this phenomenon remain unknown. In this study, we designed an optical system for evaluating spatiotem-poral changes in tissue oxygen tension (pO 2) by phosphorescence quenching. The system can measure continuous changes in pO 2 at a fixed point and can also perform two-dimensional mapping of pO 2 in any part of the tumor tissue. We implanted tumor tissue in a dorsal skinfold chamber of C57BL/6 mice and observed tumor growth. After the tumor attained a diameter of 2 mm, the mice received oxygen inhalation and pO 2 was measured. Tumor pO 2 increased after inhalation but the oxygen level was not maintained despite continuous inhalation of pure oxygen; the tumor returned to a hypoxic state. These results mimic the clinical experience of oxygen inhalation treatment in radiation therapy. Our system reproduces the repeat hypoxic phenomenon in a murine tumor model and can be used to determine the mechanisms of oxygen metabolism in tumors.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages5965-5968
Number of pages4
DOIs
Publication statusPublished - 2011
Event33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, United States
Duration: 2011 Aug 302011 Sep 3

Other

Other33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
CountryUnited States
CityBoston, MA
Period11/8/3011/9/3

Fingerprint

Oxygenation
Tumors
Oxygen
Inhalation
Neoplasms
Radiotherapy
Tissue
Optical Devices
Phosphorescence
Inbred C57BL Mouse
Metabolism
Optical systems
Quenching

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Yamada, R., Horinouch, H., & Tsukada, K. (2011). Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 5965-5968). [6091474] https://doi.org/10.1109/IEMBS.2011.6091474

Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice. / Yamada, Ryo; Horinouch, Hirohisa; Tsukada, Kosuke.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2011. p. 5965-5968 6091474.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamada, R, Horinouch, H & Tsukada, K 2011, Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6091474, pp. 5965-5968, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011, Boston, MA, United States, 11/8/30. https://doi.org/10.1109/IEMBS.2011.6091474
Yamada R, Horinouch H, Tsukada K. Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2011. p. 5965-5968. 6091474 https://doi.org/10.1109/IEMBS.2011.6091474
Yamada, Ryo ; Horinouch, Hirohisa ; Tsukada, Kosuke. / Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2011. pp. 5965-5968
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