Spatiotemporal patterns of Musashil expression during inner ear development

Hirofumi Sakaguchi; Takeshi Yaoi; Toshihiro Suzuki; Hideyuki Okano; Yasuo Hisa; Shinji Fushiki

doi:10.1097/00001756-200404290-00013

Spatiotemporal patterns of Musashil expression during inner ear development

Hirofumi Sakaguchi, Takeshi Yaoi, Toshihiro Suzuki, Hideyuki Okano, Yasuo Hisa, Shinji Fushiki

Department of Physiology

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Musashil (Msi I) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells.To investigate the involvement of MsiI in the inner ear development, we studied the expression of MsiI in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that MsiI was expressed in all otocyst cells at embryonic day (E) 10 and 12. MsiI immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of MsiI changed from "cytoplasmic predominance" to "nuclear predominance" during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.

Original language	English
Pages (from-to)	997-1001
Number of pages	5
Journal	NeuroReport
Volume	15
Issue number	6
DOIs	https://doi.org/10.1097/00001756-200404290-00013
Publication status	Published - 2004 Apr 29

Keywords

Cochlea
Development
Hair cells
Immunohistochemistry
Inner ear
Musashil
RNA binding protein
RT-PCR
Supporting cells
Vestibule

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-200404290-00013

Cite this

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abstract = "Musashil (Msi I) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells.To investigate the involvement of MsiI in the inner ear development, we studied the expression of MsiI in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that MsiI was expressed in all otocyst cells at embryonic day (E) 10 and 12. MsiI immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of MsiI changed from {"}cytoplasmic predominance{"} to {"}nuclear predominance{"} during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.",

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author = "Hirofumi Sakaguchi and Takeshi Yaoi and Toshihiro Suzuki and Hideyuki Okano and Yasuo Hisa and Shinji Fushiki",

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T1 - Spatiotemporal patterns of Musashil expression during inner ear development

AU - Sakaguchi, Hirofumi

AU - Yaoi, Takeshi

AU - Suzuki, Toshihiro

AU - Okano, Hideyuki

AU - Hisa, Yasuo

AU - Fushiki, Shinji

PY - 2004/4/29

Y1 - 2004/4/29

N2 - Musashil (Msi I) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells.To investigate the involvement of MsiI in the inner ear development, we studied the expression of MsiI in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that MsiI was expressed in all otocyst cells at embryonic day (E) 10 and 12. MsiI immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of MsiI changed from "cytoplasmic predominance" to "nuclear predominance" during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.

AB - Musashil (Msi I) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells.To investigate the involvement of MsiI in the inner ear development, we studied the expression of MsiI in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that MsiI was expressed in all otocyst cells at embryonic day (E) 10 and 12. MsiI immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of MsiI changed from "cytoplasmic predominance" to "nuclear predominance" during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.

KW - Cochlea

KW - Development

KW - Hair cells

KW - Immunohistochemistry

KW - Inner ear

KW - Musashil

KW - RNA binding protein

KW - RT-PCR

KW - Supporting cells

KW - Vestibule

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DO - 10.1097/00001756-200404290-00013

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AN - SCOPUS:2342629355

SN - 0959-4965

VL - 15

SP - 997

EP - 1001

JO - NeuroReport

JF - NeuroReport

IS - 6

ER -

Spatiotemporal patterns of Musashil expression during inner ear development

Abstract

Keywords

ASJC Scopus subject areas

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