Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice

Chikako Shimokawa, Richard Culleton, Takashi Imai, Kazutomo Suzue, Makoto Hirai, Tomoyo Taniguchi, Seiki Kobayashi, Hajime Hisaeda, Shinjiro Hamano

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Entamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. There is currently no vaccine against this parasite. We have previously shown that intracecal inoculation of E. histolytica trophozoites leads to chronic and non-healing cecitis in mice. Entamoeba moshkovskii, a closely related amoeba, also causes diarrhea and other intestinal disorders in this model. Here, we investigated the effect of infection followed by drug-cure of these species on the induction of immunity against homologous or heterologous species challenge. Mice were infected with E. histolytica or E. moshkovskii and treated with metronidazole 14 days later. Re-challenge with E. histolytica or E. moshkovskii was conducted seven or 28 days following confirmation of the clearance of amoebae, and the degree of protection compared to non-exposed control mice was evaluated. We show that primary infection with these amoebae induces a species-specific immune response which protects against challenge with the homologous, but not a heterologous species. These findings pave the way, therefore, for the identification of novel amoebae antigens that may become the targets of vaccines and provide a useful platform to investigate host protective immunity to Entamoeba infections.

Original languageEnglish
Article numbere82025
JournalPLoS One
Volume8
Issue number11
DOIs
Publication statusPublished - 2013 Nov 29

Fingerprint

Entamoeba
Entamoeba histolytica
Amoeba
Immunity
Vaccines
immunity
Metronidazole
mice
Infection
infection
Antigens
Typhlitis
amebiasis
vaccines
metronidazole
Amebiasis
Trophozoites
trophozoites
Pharmaceutical Preparations
digestive system diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shimokawa, C., Culleton, R., Imai, T., Suzue, K., Hirai, M., Taniguchi, T., ... Hamano, S. (2013). Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. PLoS One, 8(11), [e82025]. https://doi.org/10.1371/journal.pone.0082025

Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. / Shimokawa, Chikako; Culleton, Richard; Imai, Takashi; Suzue, Kazutomo; Hirai, Makoto; Taniguchi, Tomoyo; Kobayashi, Seiki; Hisaeda, Hajime; Hamano, Shinjiro.

In: PLoS One, Vol. 8, No. 11, e82025, 29.11.2013.

Research output: Contribution to journalArticle

Shimokawa, C, Culleton, R, Imai, T, Suzue, K, Hirai, M, Taniguchi, T, Kobayashi, S, Hisaeda, H & Hamano, S 2013, 'Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice', PLoS One, vol. 8, no. 11, e82025. https://doi.org/10.1371/journal.pone.0082025
Shimokawa, Chikako ; Culleton, Richard ; Imai, Takashi ; Suzue, Kazutomo ; Hirai, Makoto ; Taniguchi, Tomoyo ; Kobayashi, Seiki ; Hisaeda, Hajime ; Hamano, Shinjiro. / Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. In: PLoS One. 2013 ; Vol. 8, No. 11.
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