Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells

Yoshimasa Saito, Gangning Liang, Gerda Egger, Jeffrey M. Friedman, Jody C. Chuang, Gerhard A. Coetzee, Peter A. Jones

Research output: Contribution to journalArticle

1081 Citations (Scopus)

Abstract

Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2′-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.

Original languageEnglish
Pages (from-to)435-443
Number of pages9
JournalCancer Cell
Volume9
Issue number6
DOIs
Publication statusPublished - 2006 Jun 13
Externally publishedYes

Keywords

  • CELLCYCLE
  • DNA
  • RNA

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

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