Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome

Kuniaki Saito, Kazumichi M. Nishida, Tomoko Mori, Yoshinori Kawamura, Keita Miyoshi, Tomoko Nagami, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

418 Citations (Scopus)

Abstract

In Drosophila, Piwi (P-element-induced wimpy testis), which encodes a protein of the Argonaute family, is essential for germ stem cell self-renewal. Piwi has recently been shown to be a nuclear protein involved in gene silencing of retrotransposons and controlling their mobilization in the male germline. However, little is known about the molecular mechanisms of Piwi-dependent gene silencing. Here we show that endogenous Piwi immunopurified from ovary specifically associates with small RNAs of 25-29 nucleotides in length. Piwi-associated small RNAs were identified by cloning and sequencing as repeat-associated small interfering RNAs (rasiRNAs) derived from repetitive regions, such as retrotransposon and heterochromatic regions, in the Drosophila genome. Northern blot analyses revealed that in vivo Piwi does not associate with microRNAs (miRNAs) and that guide siRNA was not loaded onto Piwi when siRNA duplex was added to ovary lysate. In vitro, recombinant Piwi exhibits target RNA cleavage activity. These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets.

Original languageEnglish
Pages (from-to)2214-2222
Number of pages9
JournalGenes and Development
Volume20
Issue number16
DOIs
Publication statusPublished - 2006 Aug 15
Externally publishedYes

Fingerprint

Retroelements
Small Interfering RNA
Drosophila
Testis
Genome
Gene Silencing
Ovary
Argonaute Proteins
Nucleic Acid Repetitive Sequences
Nuclear Proteins
MicroRNAs
Germ Cells
Northern Blotting
Organism Cloning
Nucleotides

Keywords

  • Drosophila
  • Piwi
  • rasiRNA
  • Retrotransposon
  • Slicer

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Saito, K., Nishida, K. M., Mori, T., Kawamura, Y., Miyoshi, K., Nagami, T., ... Siomi, M. C. (2006). Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome. Genes and Development, 20(16), 2214-2222. https://doi.org/10.1101/gad.1454806

Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome. / Saito, Kuniaki; Nishida, Kazumichi M.; Mori, Tomoko; Kawamura, Yoshinori; Miyoshi, Keita; Nagami, Tomoko; Siomi, Haruhiko; Siomi, Mikiko C.

In: Genes and Development, Vol. 20, No. 16, 15.08.2006, p. 2214-2222.

Research output: Contribution to journalArticle

Saito, Kuniaki ; Nishida, Kazumichi M. ; Mori, Tomoko ; Kawamura, Yoshinori ; Miyoshi, Keita ; Nagami, Tomoko ; Siomi, Haruhiko ; Siomi, Mikiko C. / Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome. In: Genes and Development. 2006 ; Vol. 20, No. 16. pp. 2214-2222.
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