Specific detection of anti-cell surface antibodies in herpes gestationis sera

T. Hashimoto, Masayuki Amagai, H. Murakami, M. Higashiyama, K. Hashimoto, T. Nishikawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We examined 42 herpes gestationis sera with immunofluorescence of normal human skin sections, and found that anti-keratinocyte cell surface antibodies were detected specifically in 10 herpes gestationis sera. The diagnosis of these herpes gestationis cases was confirmed by detecting antibodies against the 180 kD bullous pemphigoid antigen with immunoblotting of its fusion protein. The results of immunoadsorption assay using baculoproteins of both pemphigus vulgaris and pemphigus foliaceus antigens indicated that the herpes gestationis sera did not recognize common pemphigus antigens. Immunoblotting of human epidermal extracts and immunofluorescence of various tissues also suggested that the sera did not recognize any other desmosomal components or paraneoplastic pemphigus antigens. The significance of this reactivity is unclear. However, because no control bullous pemphigoid sera showed this reactivity, it may suggest a different pathophysiology between herpes gestationis and bullous pemphigoid.

Original languageEnglish
Pages (from-to)96-101
Number of pages6
JournalExperimental Dermatology
Volume5
Issue number2
DOIs
Publication statusPublished - 1996

Fingerprint

Pemphigoid Gestationis
Bullous Pemphigoid
Pemphigus
Antigens
Antibodies
Desmoglein 1
Serum
Immunoblotting
Fluorescent Antibody Technique
Assays
Skin
Fusion reactions
Tissue
Keratinocytes
Proteins

Keywords

  • Autoantigen
  • Bullous pemphigoid
  • Keratinocyte
  • Pemphigus

ASJC Scopus subject areas

  • Dermatology

Cite this

Specific detection of anti-cell surface antibodies in herpes gestationis sera. / Hashimoto, T.; Amagai, Masayuki; Murakami, H.; Higashiyama, M.; Hashimoto, K.; Nishikawa, T.

In: Experimental Dermatology, Vol. 5, No. 2, 1996, p. 96-101.

Research output: Contribution to journalArticle

Hashimoto, T, Amagai, M, Murakami, H, Higashiyama, M, Hashimoto, K & Nishikawa, T 1996, 'Specific detection of anti-cell surface antibodies in herpes gestationis sera', Experimental Dermatology, vol. 5, no. 2, pp. 96-101. https://doi.org/10.1111/j.1600-0625.1996.tb00101.x
Hashimoto, T. ; Amagai, Masayuki ; Murakami, H. ; Higashiyama, M. ; Hashimoto, K. ; Nishikawa, T. / Specific detection of anti-cell surface antibodies in herpes gestationis sera. In: Experimental Dermatology. 1996 ; Vol. 5, No. 2. pp. 96-101.
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