Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin

Hidenori Sato, Kazuki Takeda, Koji Tani, Tomoya Hino, Tetsuji Okada, Masayoshi Nakasako, Nobuo Kamiya, Tsutomu Kouyama

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In the purple membrane of Halobacterium salinarium, bacteriorhodopsin trimers are arranged in a hexagonal lattice. When purple membrane sheets are incubated at high temperature with neutral detergent, membrane vesicularization takes place, yielding inside-out vesicles with a diameter of 50 nm. The vesicular structure becomes unstable at low temperature, where successive fusion of the vesicles yields a crystal which is composed of stacked planar membranes. X-ray crystallographic analysis reveals that the bacteriorhodopsin trimers are arranged in a honeycomb lattice in each membrane layer and that neighbouring membranes orient in opposite directions. The native structure of the trimeric unit is preserved in the honeycomb lattice, irrespective of alterations in the in-plane orientation of the trimer. One phospholipid tightly bound to a crevice between monomers in the trimeric unit is suggested to act as a glue in the formation of the trimer.

Original languageEnglish
Pages (from-to)1251-1256
Number of pages6
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number7
DOIs
Publication statusPublished - 1999 Jul
Externally publishedYes

Fingerprint

Bacteriorhodopsins
lipids
Purple Membrane
trimers
membranes
proteins
Membranes
Lipids
Proteins
Halobacterium
interactions
Temperature
Adhesives
Detergents
Phospholipids
glues
detergents
Glues
X-Rays
Fusion reactions

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin. / Sato, Hidenori; Takeda, Kazuki; Tani, Koji; Hino, Tomoya; Okada, Tetsuji; Nakasako, Masayoshi; Kamiya, Nobuo; Kouyama, Tsutomu.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 7, 07.1999, p. 1251-1256.

Research output: Contribution to journalArticle

Sato, Hidenori ; Takeda, Kazuki ; Tani, Koji ; Hino, Tomoya ; Okada, Tetsuji ; Nakasako, Masayoshi ; Kamiya, Nobuo ; Kouyama, Tsutomu. / Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin. In: Acta Crystallographica Section D: Biological Crystallography. 1999 ; Vol. 55, No. 7. pp. 1251-1256.
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