Abstract
Human spermatozoal RNAs were recently profiled using microarrays and explored as clinical markers of male infertility. An appropriate study design with a considerable number of biological replicates (sperm samples) is necessary to validate the accuracy and reproducibility of these microarray data. If the genes identified as sperm quality markers by microarray studies are successfully attributed to the pathogenesis of male infertility, then the microarray strategy may be used as a clinical diagnostic tool for male infertility. On the other hand, spermatozoal RNAs may contain not only remnant RNAs after spermatogenesis, but also RNAs that may contribute extragenomically to early embryonic development. Therefore, spermatozoal RNA profiling may enable a better understanding of what is contributed to the oocyte by sperm, in addition to their genome, to facilitate early embryonic development.
| Original language | English |
|---|---|
| Pages (from-to) | 103-105 |
| Number of pages | 3 |
| Journal | Reproductive BioMedicine Online |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Feb |
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Keywords
- ICSI
- male fertility
- RNA profiling
- sperm
- spermatozoa
- transcriptome
ASJC Scopus subject areas
- Reproductive Medicine
- Developmental Biology
Cite this
Spermatozoal RNA profiling towards a clinical evaluation of sperm quality. / Hamatani, Toshio.
In: Reproductive BioMedicine Online, Vol. 22, No. 2, 02.2011, p. 103-105.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spermatozoal RNA profiling towards a clinical evaluation of sperm quality
AU - Hamatani, Toshio
PY - 2011/2
Y1 - 2011/2
N2 - Human spermatozoal RNAs were recently profiled using microarrays and explored as clinical markers of male infertility. An appropriate study design with a considerable number of biological replicates (sperm samples) is necessary to validate the accuracy and reproducibility of these microarray data. If the genes identified as sperm quality markers by microarray studies are successfully attributed to the pathogenesis of male infertility, then the microarray strategy may be used as a clinical diagnostic tool for male infertility. On the other hand, spermatozoal RNAs may contain not only remnant RNAs after spermatogenesis, but also RNAs that may contribute extragenomically to early embryonic development. Therefore, spermatozoal RNA profiling may enable a better understanding of what is contributed to the oocyte by sperm, in addition to their genome, to facilitate early embryonic development.
AB - Human spermatozoal RNAs were recently profiled using microarrays and explored as clinical markers of male infertility. An appropriate study design with a considerable number of biological replicates (sperm samples) is necessary to validate the accuracy and reproducibility of these microarray data. If the genes identified as sperm quality markers by microarray studies are successfully attributed to the pathogenesis of male infertility, then the microarray strategy may be used as a clinical diagnostic tool for male infertility. On the other hand, spermatozoal RNAs may contain not only remnant RNAs after spermatogenesis, but also RNAs that may contribute extragenomically to early embryonic development. Therefore, spermatozoal RNA profiling may enable a better understanding of what is contributed to the oocyte by sperm, in addition to their genome, to facilitate early embryonic development.
KW - ICSI
KW - male fertility
KW - RNA profiling
KW - sperm
KW - spermatozoa
KW - transcriptome
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U2 - 10.1016/j.rbmo.2010.12.001
DO - 10.1016/j.rbmo.2010.12.001
M3 - Article
VL - 22
SP - 103
EP - 105
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 2
ER -