Sphingosine Kinase Type 1 Induces G12/13-mediated Stress Fiber Formation, yet Promotes Growth and Survival Independent of G Protein-coupled Receptors

Ana Olivera, Hans M. Rosenfeldt, Meryem Bektas, Fang Wang, Isao Ishii, Jerold Chun, Sheldon Milstien, Sarah Spiegel

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Sphingosine 1-phosphate (S1P) is the ligand for a family of specific G protein-coupled receptors (GPCRs) that regulate a wide variety of important cellular functions, including growth, survival, cytoskeletal rearrangements, and cell motility. However, whether it also has an intracellular function is still a matter of great debate. Overexpression of sphingosine kinase type 1, which generated S1P, induced extensive stress fibers and impaired formation of the Src-focal adhesion kinase signaling complex, with consequent aberrant focal adhesion turnover, leading to inhibition of cell locomotion. We have dissected biological responses dependent on intracellular S1P from those that are receptor-mediated by specifically blocking signaling of Gαq, Gαi, Gα12/13, and Gβγ subunits, the G proteins that S1P receptors (S1PRs) couple to and signal through. We found that intracellular S1P signaled "inside out" through its cell-surface receptors linked to G12/13-mediated stress fiber formation, important for cell motility. Remarkably, cell growth stimulation and suppression of apoptosis by endogenous S1P were independent of GPCRs and inside-out signaling. Using fibroblasts from embryonic mice devoid of functional S1PRs, we also demonstrated that, in contrast to exogenous S1P, intracellular S1P formed by overexpression of sphingosine kinase type 1 promoted growth and survival independent of its GPCRs. Hence, exogenous and intracellularly generated S1Ps affect cell growth and survival by divergent pathways. Our results demonstrate a receptor-independent intracellular function of S1P, reminiscent of its action in yeast cells that lack S1PRs.

Original languageEnglish
Pages (from-to)46452-46460
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number47
DOIs
Publication statusPublished - 2003 Nov 21
Externally publishedYes

Fingerprint

Stress Fibers
G-Protein-Coupled Receptors
Lysosphingolipid Receptors
Fibers
Growth
Cell Movement
Cell growth
Cells
Focal Adhesion Protein-Tyrosine Kinases
Focal Adhesions
Cell Surface Receptors
Fibroblasts
sphingosine 1-phosphate
sphingosine kinase
GTP-Binding Proteins
Yeast
Cell Survival
Adhesion
Yeasts
Apoptosis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sphingosine Kinase Type 1 Induces G12/13-mediated Stress Fiber Formation, yet Promotes Growth and Survival Independent of G Protein-coupled Receptors. / Olivera, Ana; Rosenfeldt, Hans M.; Bektas, Meryem; Wang, Fang; Ishii, Isao; Chun, Jerold; Milstien, Sheldon; Spiegel, Sarah.

In: Journal of Biological Chemistry, Vol. 278, No. 47, 21.11.2003, p. 46452-46460.

Research output: Contribution to journalArticle

Olivera, Ana ; Rosenfeldt, Hans M. ; Bektas, Meryem ; Wang, Fang ; Ishii, Isao ; Chun, Jerold ; Milstien, Sheldon ; Spiegel, Sarah. / Sphingosine Kinase Type 1 Induces G12/13-mediated Stress Fiber Formation, yet Promotes Growth and Survival Independent of G Protein-coupled Receptors. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 47. pp. 46452-46460.
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