Spike-dependent calcium influx in dendrites of the cricket giant interneuron

Hiroto Ogawa, Yoshichika Baba, Kotaro Oka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Identified wind-sensitive giant interneurons in the cricket's cercal sensory system integrate cercal afferent signals and release an avoidance behavior. A calcium-imaging technique was applied to the giant interneurons to examine the presence of the voltage-dependent Ca2+ channels (VDCCs) in their dendrites. We found that presynaptic stimuli to the cercal sensory nerve cords elevated the cytosolic Ca2+ concentration ([Ca2+](i) in the dendrites of the giant interneurons. The dendritic Ca2+ rise coincided with the spike burst of the giant interneurons, and the rate of Ca2+ rise depended on the frequency of the action potentials. These results suggest that the action potentials directly caused [Ca2+](i) increase. Observation of the [Ca2+](i) elevation induced by depolarizing current injection demonstrates the presence of the VDCCs in the dendrites. Although hyperpolarizing current injection into the giant interneuron suppressed action potential generation, EPSPs could induce no [Ca2+](i) increase. This result means that ligand-gated channels do not contribute to the synaptically stimulated Ca2+ elevation. On the other hand, antidromically stimulated spikes also increased [Ca2+](i) in all cellular regions including the dendrites. And bath application of a mixture of Ni2+, Co2+, and Cd2+ or tetrodotoxin inhibited the [Ca2+](i) elevation induced by the antidromic stimulation. From these findings, we suppose that the axonal spikes antidromically propagate and induce the Ca2+ influx via VDCCs in the dendrites. The spike-dependent Ca2+ elevation may regulate the sensory signals processing via second-messenger cascades in the giant interneurons. (C) 2000 John Wiley and Sons. Inc.

Original languageEnglish
Pages (from-to)45-56
Number of pages12
JournalJournal of Neurobiology
Volume44
Issue number1
DOIs
Publication statusPublished - 2000

Fingerprint

Gryllidae
Calcium Signaling
Interneurons
Dendrites
Action Potentials
Avoidance Learning
Ligand-Gated Ion Channels
Injections
Excitatory Postsynaptic Potentials
Tetrodotoxin
Second Messenger Systems
Baths
Observation
Calcium

Keywords

  • Active dendrite
  • Ca imaging
  • Calcium Green-1
  • Cricket
  • Giant interneuron
  • Voltage-dependent Ca channel

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spike-dependent calcium influx in dendrites of the cricket giant interneuron. / Ogawa, Hiroto; Baba, Yoshichika; Oka, Kotaro.

In: Journal of Neurobiology, Vol. 44, No. 1, 2000, p. 45-56.

Research output: Contribution to journalArticle

Ogawa, Hiroto ; Baba, Yoshichika ; Oka, Kotaro. / Spike-dependent calcium influx in dendrites of the cricket giant interneuron. In: Journal of Neurobiology. 2000 ; Vol. 44, No. 1. pp. 45-56.
@article{13b63333050848ee83d9f3c18c486afe,
title = "Spike-dependent calcium influx in dendrites of the cricket giant interneuron",
abstract = "Identified wind-sensitive giant interneurons in the cricket's cercal sensory system integrate cercal afferent signals and release an avoidance behavior. A calcium-imaging technique was applied to the giant interneurons to examine the presence of the voltage-dependent Ca2+ channels (VDCCs) in their dendrites. We found that presynaptic stimuli to the cercal sensory nerve cords elevated the cytosolic Ca2+ concentration ([Ca2+](i) in the dendrites of the giant interneurons. The dendritic Ca2+ rise coincided with the spike burst of the giant interneurons, and the rate of Ca2+ rise depended on the frequency of the action potentials. These results suggest that the action potentials directly caused [Ca2+](i) increase. Observation of the [Ca2+](i) elevation induced by depolarizing current injection demonstrates the presence of the VDCCs in the dendrites. Although hyperpolarizing current injection into the giant interneuron suppressed action potential generation, EPSPs could induce no [Ca2+](i) increase. This result means that ligand-gated channels do not contribute to the synaptically stimulated Ca2+ elevation. On the other hand, antidromically stimulated spikes also increased [Ca2+](i) in all cellular regions including the dendrites. And bath application of a mixture of Ni2+, Co2+, and Cd2+ or tetrodotoxin inhibited the [Ca2+](i) elevation induced by the antidromic stimulation. From these findings, we suppose that the axonal spikes antidromically propagate and induce the Ca2+ influx via VDCCs in the dendrites. The spike-dependent Ca2+ elevation may regulate the sensory signals processing via second-messenger cascades in the giant interneurons. (C) 2000 John Wiley and Sons. Inc.",
keywords = "Active dendrite, Ca imaging, Calcium Green-1, Cricket, Giant interneuron, Voltage-dependent Ca channel",
author = "Hiroto Ogawa and Yoshichika Baba and Kotaro Oka",
year = "2000",
doi = "10.1002/1097-4695(200007)44:1<45::AID-NEU5>3.0.CO;2-#",
language = "English",
volume = "44",
pages = "45--56",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Spike-dependent calcium influx in dendrites of the cricket giant interneuron

AU - Ogawa, Hiroto

AU - Baba, Yoshichika

AU - Oka, Kotaro

PY - 2000

Y1 - 2000

N2 - Identified wind-sensitive giant interneurons in the cricket's cercal sensory system integrate cercal afferent signals and release an avoidance behavior. A calcium-imaging technique was applied to the giant interneurons to examine the presence of the voltage-dependent Ca2+ channels (VDCCs) in their dendrites. We found that presynaptic stimuli to the cercal sensory nerve cords elevated the cytosolic Ca2+ concentration ([Ca2+](i) in the dendrites of the giant interneurons. The dendritic Ca2+ rise coincided with the spike burst of the giant interneurons, and the rate of Ca2+ rise depended on the frequency of the action potentials. These results suggest that the action potentials directly caused [Ca2+](i) increase. Observation of the [Ca2+](i) elevation induced by depolarizing current injection demonstrates the presence of the VDCCs in the dendrites. Although hyperpolarizing current injection into the giant interneuron suppressed action potential generation, EPSPs could induce no [Ca2+](i) increase. This result means that ligand-gated channels do not contribute to the synaptically stimulated Ca2+ elevation. On the other hand, antidromically stimulated spikes also increased [Ca2+](i) in all cellular regions including the dendrites. And bath application of a mixture of Ni2+, Co2+, and Cd2+ or tetrodotoxin inhibited the [Ca2+](i) elevation induced by the antidromic stimulation. From these findings, we suppose that the axonal spikes antidromically propagate and induce the Ca2+ influx via VDCCs in the dendrites. The spike-dependent Ca2+ elevation may regulate the sensory signals processing via second-messenger cascades in the giant interneurons. (C) 2000 John Wiley and Sons. Inc.

AB - Identified wind-sensitive giant interneurons in the cricket's cercal sensory system integrate cercal afferent signals and release an avoidance behavior. A calcium-imaging technique was applied to the giant interneurons to examine the presence of the voltage-dependent Ca2+ channels (VDCCs) in their dendrites. We found that presynaptic stimuli to the cercal sensory nerve cords elevated the cytosolic Ca2+ concentration ([Ca2+](i) in the dendrites of the giant interneurons. The dendritic Ca2+ rise coincided with the spike burst of the giant interneurons, and the rate of Ca2+ rise depended on the frequency of the action potentials. These results suggest that the action potentials directly caused [Ca2+](i) increase. Observation of the [Ca2+](i) elevation induced by depolarizing current injection demonstrates the presence of the VDCCs in the dendrites. Although hyperpolarizing current injection into the giant interneuron suppressed action potential generation, EPSPs could induce no [Ca2+](i) increase. This result means that ligand-gated channels do not contribute to the synaptically stimulated Ca2+ elevation. On the other hand, antidromically stimulated spikes also increased [Ca2+](i) in all cellular regions including the dendrites. And bath application of a mixture of Ni2+, Co2+, and Cd2+ or tetrodotoxin inhibited the [Ca2+](i) elevation induced by the antidromic stimulation. From these findings, we suppose that the axonal spikes antidromically propagate and induce the Ca2+ influx via VDCCs in the dendrites. The spike-dependent Ca2+ elevation may regulate the sensory signals processing via second-messenger cascades in the giant interneurons. (C) 2000 John Wiley and Sons. Inc.

KW - Active dendrite

KW - Ca imaging

KW - Calcium Green-1

KW - Cricket

KW - Giant interneuron

KW - Voltage-dependent Ca channel

UR - http://www.scopus.com/inward/record.url?scp=0033933481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033933481&partnerID=8YFLogxK

U2 - 10.1002/1097-4695(200007)44:1<45::AID-NEU5>3.0.CO;2-#

DO - 10.1002/1097-4695(200007)44:1<45::AID-NEU5>3.0.CO;2-#

M3 - Article

VL - 44

SP - 45

EP - 56

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 1

ER -