Spike-triggered dendritic calcium transients depend on synaptic activity in the cricket giant interneurons

Hiroto Ogawa, Yoshichika Baba, Kotaro Oka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The relationship between electrical activity and spike-induced Ca2+ increases in dendrites was investigated in the identified wind-sensitive giant interneurons in the cricket. We applied a high-speed Ca2+ imaging technique to the giant interneurons, and succeeded in recording the transient Ca2+ increases (Ca2+ transients) induced by a single action potential, which was evoked by presynaptic stimulus to the sensory neurons. The dendritic Ca2+ transients evoked by a pair of action potentials accumulated when spike intervals were shorter than 100 ms. The amplitude of the Ca2+ transients induced by a train of spikes depended on the number of action potentials. When stimulation pulses evoking the same numbers of action potentials were separately applied to the ipsi- or contra-lateral cercal sensory nerves, the dendritic Ca2+ transients induced by these presynaptic stimuli were different in their amplitude. Furthermore, the side of presynaptic stimulation that evoked larger Ca2+ transients depended on the location of the recorded dendritic regions. This result means that the spike-triggered Ca2+ transients in dendrites depend on postsynaptic activity. It is proposed that Ca2+ entry through voltage-dependent Ca2+ channels activated by the action potentials will be enhanced by excitatory synaptic inputs at the dendrites in the cricket giant interneurons.

Original languageEnglish
Pages (from-to)234-244
Number of pages11
JournalJournal of Neurobiology
Volume50
Issue number3
DOIs
Publication statusPublished - 2002 Feb 15

Fingerprint

Gryllidae
Interneurons
Action Potentials
Calcium
Dendrites
Sensory Receptor Cells

Keywords

  • Ca imaging
  • Cricket
  • Dendrite
  • Giant interneuron
  • Oregon Green 488 BAPTA-1

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spike-triggered dendritic calcium transients depend on synaptic activity in the cricket giant interneurons. / Ogawa, Hiroto; Baba, Yoshichika; Oka, Kotaro.

In: Journal of Neurobiology, Vol. 50, No. 3, 15.02.2002, p. 234-244.

Research output: Contribution to journalArticle

@article{5639391977cf4d768c36a7d1a0622f71,
title = "Spike-triggered dendritic calcium transients depend on synaptic activity in the cricket giant interneurons",
abstract = "The relationship between electrical activity and spike-induced Ca2+ increases in dendrites was investigated in the identified wind-sensitive giant interneurons in the cricket. We applied a high-speed Ca2+ imaging technique to the giant interneurons, and succeeded in recording the transient Ca2+ increases (Ca2+ transients) induced by a single action potential, which was evoked by presynaptic stimulus to the sensory neurons. The dendritic Ca2+ transients evoked by a pair of action potentials accumulated when spike intervals were shorter than 100 ms. The amplitude of the Ca2+ transients induced by a train of spikes depended on the number of action potentials. When stimulation pulses evoking the same numbers of action potentials were separately applied to the ipsi- or contra-lateral cercal sensory nerves, the dendritic Ca2+ transients induced by these presynaptic stimuli were different in their amplitude. Furthermore, the side of presynaptic stimulation that evoked larger Ca2+ transients depended on the location of the recorded dendritic regions. This result means that the spike-triggered Ca2+ transients in dendrites depend on postsynaptic activity. It is proposed that Ca2+ entry through voltage-dependent Ca2+ channels activated by the action potentials will be enhanced by excitatory synaptic inputs at the dendrites in the cricket giant interneurons.",
keywords = "Ca imaging, Cricket, Dendrite, Giant interneuron, Oregon Green 488 BAPTA-1",
author = "Hiroto Ogawa and Yoshichika Baba and Kotaro Oka",
year = "2002",
month = "2",
day = "15",
doi = "10.1002/neu.10032",
language = "English",
volume = "50",
pages = "234--244",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Spike-triggered dendritic calcium transients depend on synaptic activity in the cricket giant interneurons

AU - Ogawa, Hiroto

AU - Baba, Yoshichika

AU - Oka, Kotaro

PY - 2002/2/15

Y1 - 2002/2/15

N2 - The relationship between electrical activity and spike-induced Ca2+ increases in dendrites was investigated in the identified wind-sensitive giant interneurons in the cricket. We applied a high-speed Ca2+ imaging technique to the giant interneurons, and succeeded in recording the transient Ca2+ increases (Ca2+ transients) induced by a single action potential, which was evoked by presynaptic stimulus to the sensory neurons. The dendritic Ca2+ transients evoked by a pair of action potentials accumulated when spike intervals were shorter than 100 ms. The amplitude of the Ca2+ transients induced by a train of spikes depended on the number of action potentials. When stimulation pulses evoking the same numbers of action potentials were separately applied to the ipsi- or contra-lateral cercal sensory nerves, the dendritic Ca2+ transients induced by these presynaptic stimuli were different in their amplitude. Furthermore, the side of presynaptic stimulation that evoked larger Ca2+ transients depended on the location of the recorded dendritic regions. This result means that the spike-triggered Ca2+ transients in dendrites depend on postsynaptic activity. It is proposed that Ca2+ entry through voltage-dependent Ca2+ channels activated by the action potentials will be enhanced by excitatory synaptic inputs at the dendrites in the cricket giant interneurons.

AB - The relationship between electrical activity and spike-induced Ca2+ increases in dendrites was investigated in the identified wind-sensitive giant interneurons in the cricket. We applied a high-speed Ca2+ imaging technique to the giant interneurons, and succeeded in recording the transient Ca2+ increases (Ca2+ transients) induced by a single action potential, which was evoked by presynaptic stimulus to the sensory neurons. The dendritic Ca2+ transients evoked by a pair of action potentials accumulated when spike intervals were shorter than 100 ms. The amplitude of the Ca2+ transients induced by a train of spikes depended on the number of action potentials. When stimulation pulses evoking the same numbers of action potentials were separately applied to the ipsi- or contra-lateral cercal sensory nerves, the dendritic Ca2+ transients induced by these presynaptic stimuli were different in their amplitude. Furthermore, the side of presynaptic stimulation that evoked larger Ca2+ transients depended on the location of the recorded dendritic regions. This result means that the spike-triggered Ca2+ transients in dendrites depend on postsynaptic activity. It is proposed that Ca2+ entry through voltage-dependent Ca2+ channels activated by the action potentials will be enhanced by excitatory synaptic inputs at the dendrites in the cricket giant interneurons.

KW - Ca imaging

KW - Cricket

KW - Dendrite

KW - Giant interneuron

KW - Oregon Green 488 BAPTA-1

UR - http://www.scopus.com/inward/record.url?scp=0037083215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037083215&partnerID=8YFLogxK

U2 - 10.1002/neu.10032

DO - 10.1002/neu.10032

M3 - Article

VL - 50

SP - 234

EP - 244

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 3

ER -