Split-and-contact device to form planar lipid bilayers

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages161-164
Number of pages4
DOIs
Publication statusPublished - 2013
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

Fingerprint

Lipid bilayers
lipids
Lipids
Membrane Lipids
Monolayers
sliding
Hemolysin Proteins
membranes
Single-Stranded DNA
Cyclodextrins
Contacts (fluid mechanics)
Organic solvents
Oils
DNA
metal spinning
drugs
deoxyribonucleic acid
oils
Water
Sensors

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Tsuji, Y., Kawano, R., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2013). Split-and-contact device to form planar lipid bilayers. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 161-164). [6474202] https://doi.org/10.1109/MEMSYS.2013.6474202

Split-and-contact device to form planar lipid bilayers. / Tsuji, Y.; Kawano, R.; Osaki, T.; Kamiya, K.; Miki, Norihisa; Takeuchi, S.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 161-164 6474202.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuji, Y, Kawano, R, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2013, Split-and-contact device to form planar lipid bilayers. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6474202, pp. 161-164, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474202
Tsuji Y, Kawano R, Osaki T, Kamiya K, Miki N, Takeuchi S. Split-and-contact device to form planar lipid bilayers. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 161-164. 6474202 https://doi.org/10.1109/MEMSYS.2013.6474202
Tsuji, Y. ; Kawano, R. ; Osaki, T. ; Kamiya, K. ; Miki, Norihisa ; Takeuchi, S. / Split-and-contact device to form planar lipid bilayers. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. pp. 161-164
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