Split-and-contact device to form planar lipid bilayers

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.

Original languageEnglish
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages161-164
Number of pages4
DOIs
Publication statusPublished - 2013 Apr 2
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Tsuji, Y., Kawano, R., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2013). Split-and-contact device to form planar lipid bilayers. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 161-164). [6474202] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474202