Spontaneous apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells

Shinji Tsutsumi, Kazuhito Rokutan, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The purpose of this study was to elucidate the mechanism of spontaneous and rapid cell death of cultured gastric pit cells. Gastric pit cells have a rapid cell turnover rate in vivo. We here show that guinea pig gastric pit cells in culture undergo spontaneous and rapid apoptotic DNA fragmentation, which may represent the rapid cell turnover cycle of gastric pit cells in vivo. This spontaneous apoptotic DNA fragmentation required the presence of fetal calf serum in the culture media. Furthermore, the spontaneous apoptotic DNA fragmentation was prevented by protein synthesis and caspase inhibitors.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalDigestive Diseases and Sciences
Volume45
Issue number2
DOIs
Publication statusPublished - 2000 Mar 16

Keywords

  • Apoptosis
  • Caspase
  • Gastric mucosal cells
  • Pit cell lineage

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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