Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient

J. Koide, K. Takada, M. Sugiura, H. Sekine, T. Ito, K. Saito, S. Mori, T. Takeuchi, S. Uchida, T. Abe

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Abstract

An Epstein-Barr virus (EBV)-infected fibroblast line, designated DSEK, was spontaneously established from synovial tissue of a patient with rheumatoid arthritis (RA). DSEK cells expressed EBV nuclear antigens EBNA-1 and EBNA-2 and latent membrane protein LMP-1. Cell surface markers of DSEK cells were similar to those of EBV-negative fibroblast clones derived from synoviocytes and were negative for lymphocyte and macrophage markers. DSEK cells expressed CD44, CD58, and HLA. DR antigens and spontaneously produced interleukin-10 basic fibroblast growth factor and transforming growth factor β1. These results indicate that rheumatoid synoviocytes can be a target for EBV infection and suggest that EBV may play a role in the pathogenesis of RA.

Original languageEnglish
Pages (from-to)2478-2481
Number of pages4
JournalJournal of Virology
Volume71
Issue number3
Publication statusPublished - 1997 Feb 25

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Koide, J., Takada, K., Sugiura, M., Sekine, H., Ito, T., Saito, K., Mori, S., Takeuchi, T., Uchida, S., & Abe, T. (1997). Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient. Journal of Virology, 71(3), 2478-2481.