SPR sensor signal amplification based on dye-doped polymer particles

Hirokazu Komatsu; Makiko Miyachi; Eiji Fujii; Daniel Citterio; Koji Yamada; Yoshinobu Sato; Kazuyoshi Kurihara; Haruma Kawaguchi; Koji Suzuki

doi:10.1016/j.stam.2005.12.009

SPR sensor signal amplification based on dye-doped polymer particles

Hirokazu Komatsu, Makiko Miyachi, Eiji Fujii, Daniel Citterio, Koji Yamada, Yoshinobu Sato, Kazuyoshi Kurihara, Haruma Kawaguchi, Koji Suzuki

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A novel amplification method was prepared based on dye-doped polymer particles for SPR signals originating from antigen-antibody (BSA-anti-BSA) interactions. Coloring the particles enhances the change of SPR signals based on the change of the imaginary part of the refractive index. The signal amplification effect in the reflectance mode was 31-fold stronger compared to the effect obtained with white latex particles and the combined amplification effect due to the presence of polymer particles and the colorant was over 100-fold compared to non-amplified SPR signals originating from BSA-anti-BSA interactions. The amplification method based on the dye-doped particles is widely applicable for the analysis of antibody-antigen interactions and DNA interactions at low concentrations.

Original language	English
Pages (from-to)	150-155
Number of pages	6
Journal	Science and Technology of Advanced Materials
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/j.stam.2005.12.009
Publication status	Published - 2006 Mar
Externally published	Yes

Keywords

Absorption-based SPR
Amplification
BSA
Colored-particle
Polymer particle
Protein-protein interaction
Surface plasmon resonance

ASJC Scopus subject areas

Materials Science(all)

Access to Document

10.1016/j.stam.2005.12.009

Cite this

@article{271eab9b30a440289c264e607744bc99,

title = "SPR sensor signal amplification based on dye-doped polymer particles",

abstract = "A novel amplification method was prepared based on dye-doped polymer particles for SPR signals originating from antigen-antibody (BSA-anti-BSA) interactions. Coloring the particles enhances the change of SPR signals based on the change of the imaginary part of the refractive index. The signal amplification effect in the reflectance mode was 31-fold stronger compared to the effect obtained with white latex particles and the combined amplification effect due to the presence of polymer particles and the colorant was over 100-fold compared to non-amplified SPR signals originating from BSA-anti-BSA interactions. The amplification method based on the dye-doped particles is widely applicable for the analysis of antibody-antigen interactions and DNA interactions at low concentrations.",

keywords = "Absorption-based SPR, Amplification, BSA, Colored-particle, Polymer particle, Protein-protein interaction, Surface plasmon resonance",

author = "Hirokazu Komatsu and Makiko Miyachi and Eiji Fujii and Daniel Citterio and Koji Yamada and Yoshinobu Sato and Kazuyoshi Kurihara and Haruma Kawaguchi and Koji Suzuki",

note = "Funding Information: The authors thank the aid of the 21st Century COE Program of the Japan Society for the Promotion of Science (JSPS) and its life-conjugated chemistry (LCC) base of Keio University.",

year = "2006",

month = mar,

doi = "10.1016/j.stam.2005.12.009",

language = "English",

volume = "7",

pages = "150--155",

journal = "Science and Technology of Advanced Materials",

issn = "1468-6996",

publisher = "IOP Publishing Ltd.",

number = "2",

}

TY - JOUR

T1 - SPR sensor signal amplification based on dye-doped polymer particles

AU - Komatsu, Hirokazu

AU - Miyachi, Makiko

AU - Fujii, Eiji

AU - Citterio, Daniel

AU - Yamada, Koji

AU - Sato, Yoshinobu

AU - Kurihara, Kazuyoshi

AU - Kawaguchi, Haruma

AU - Suzuki, Koji

N1 - Funding Information: The authors thank the aid of the 21st Century COE Program of the Japan Society for the Promotion of Science (JSPS) and its life-conjugated chemistry (LCC) base of Keio University.

PY - 2006/3

Y1 - 2006/3

N2 - A novel amplification method was prepared based on dye-doped polymer particles for SPR signals originating from antigen-antibody (BSA-anti-BSA) interactions. Coloring the particles enhances the change of SPR signals based on the change of the imaginary part of the refractive index. The signal amplification effect in the reflectance mode was 31-fold stronger compared to the effect obtained with white latex particles and the combined amplification effect due to the presence of polymer particles and the colorant was over 100-fold compared to non-amplified SPR signals originating from BSA-anti-BSA interactions. The amplification method based on the dye-doped particles is widely applicable for the analysis of antibody-antigen interactions and DNA interactions at low concentrations.

AB - A novel amplification method was prepared based on dye-doped polymer particles for SPR signals originating from antigen-antibody (BSA-anti-BSA) interactions. Coloring the particles enhances the change of SPR signals based on the change of the imaginary part of the refractive index. The signal amplification effect in the reflectance mode was 31-fold stronger compared to the effect obtained with white latex particles and the combined amplification effect due to the presence of polymer particles and the colorant was over 100-fold compared to non-amplified SPR signals originating from BSA-anti-BSA interactions. The amplification method based on the dye-doped particles is widely applicable for the analysis of antibody-antigen interactions and DNA interactions at low concentrations.

KW - Absorption-based SPR

KW - Amplification

KW - BSA

KW - Colored-particle

KW - Polymer particle

KW - Protein-protein interaction

KW - Surface plasmon resonance

UR - http://www.scopus.com/inward/record.url?scp=33645930972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645930972&partnerID=8YFLogxK

U2 - 10.1016/j.stam.2005.12.009

DO - 10.1016/j.stam.2005.12.009

M3 - Article

AN - SCOPUS:33645930972

SN - 1468-6996

VL - 7

SP - 150

EP - 155

JO - Science and Technology of Advanced Materials

JF - Science and Technology of Advanced Materials

IS - 2

ER -

SPR sensor signal amplification based on dye-doped polymer particles

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this