Spred-2 Suppresses Aorta-Gonad-Mesonephros Hematopoiesis by Inhibiting MAP Kinase Activation

Ikuo Nobuhisa, Reiko Kato, Hirofumi Inoue, Makiko Takizawa, Keisuke Okita, Akihiko Yoshimura, Tetsuya Taga

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

In midgestation mouse embryos, the aorta-gonad-mesonephros (AGM) region generates hematopoietic stem cells and definitive hematopoiesis is regulated by cell-cell interaction and signaling molecules. We showed that a Ras/mitogen-activated protein (MAP) kinase signaling-specific inhibitor and a dominant negative mutant Ras blocked the production of CD45+ hematopoietic cells in embryonic day 11.5 AGM culture, indicating an essential role for the MAP kinase pathway in AGM hematopoiesis. Overexpression of the Ras/MAP kinase pathway regulator, Spred-2, in the AGM culture significantly reduced the number of CD45+ cells. In contrast, production of CD45+ cells from the AGM region of Spred-2-null mice was up-regulated as compared with wild-type littermates. Furthermore, Spred-2-deficient mice exhibited elevated hematopoietic colony formation from vascular endothelial-cadherin+ cells. These data indicate that Spred-2 functions as a negative regulator of AGM hematopoiesis by inhibiting hematopoietic cytokine signaling.

Original languageEnglish
Pages (from-to)737-742
Number of pages6
JournalJournal of Experimental Medicine
Volume199
Issue number5
DOIs
Publication statusPublished - 2004 Mar 1
Externally publishedYes

Keywords

  • AGM
  • C-Kit
  • Differentiation
  • Hematopoiesis
  • SCF

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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