Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy

Madoka Iida, Kentaro Sahashi, Naohide Kondo, Hideaki Nakatsuji, Genki Tohnai, Yutaka Tsutsumi, Seiya Noda, Ayuka Murakami, Kazunari Onodera, Yohei Okada, Masahiro Nakatochi, Yuka Tsukagoshi Okabe, Shinobu Shimizu, Masaaki Mizuno, Hiroaki Adachi, Hideyuki Okano, Gen Sobue, Masahisa Katsuno

Research output: Contribution to journalArticle

Abstract

Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disease caused by an expanded CAG repeat in the androgen receptor (AR) gene. Here, we perform a comprehensive analysis of signaling pathways in a mouse model of SBMA (AR-97Q mice) utilizing a phosphoprotein assay. We measure the levels of 17 phosphorylated proteins in spinal cord and skeletal muscle of AR-97Q mice at three stages. The level of phosphorylated Src (p-Src) is markedly increased in the spinal cords and skeletal muscles of AR-97Q mice prior to the onset. Intraperitoneal administration of a Src kinase inhibitor improves the behavioral and histopathological phenotypes of the transgenic mice. We identify p130Cas as an effector molecule of Src and show that the phosphorylated p130Cas is elevated in murine and cellular models of SBMA. These results suggest that Src kinase inhibition is a potential therapy for SBMA.

Original languageEnglish
Article number4262
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Iida, M., Sahashi, K., Kondo, N., Nakatsuji, H., Tohnai, G., Tsutsumi, Y., Noda, S., Murakami, A., Onodera, K., Okada, Y., Nakatochi, M., Tsukagoshi Okabe, Y., Shimizu, S., Mizuno, M., Adachi, H., Okano, H., Sobue, G., & Katsuno, M. (2019). Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy. Nature communications, 10(1), [4262]. https://doi.org/10.1038/s41467-019-12282-7