Stabilizing synthetic data in the DNA of living organisms

Nozomu Yachie, Yoshiaki Ohashi, Masaru Tomita

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Data-encoding synthetic DNA, inserted into the genome of a living organism, is thought to be more robust than the current media. Because the living genome is duplicated and copied into new generations, one of the merits of using DNA material is long-term data storage within heritable media. A disadvantage of this approach is that encoded data can be unexpectedly broken by mutation, deletion, and insertion of DNA, which occurs naturally during evolution and prolongation, or laboratory experiments. For this reason, several information theory-based approaches have been developed as an error check of broken DNA data in order to achieve data durability. These approaches cannot efficiently recover badly damaged data- encoding DNA. We recently developed a DNA data-storage approach based on the multiple sequence alignment method to achieve a high level of data durability. In this paper, we overview this technology and discuss strategies for optimal application of this approach.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalSystems and Synthetic Biology
Volume2
Issue number1-2
DOIs
Publication statusPublished - 2008 Jun

Fingerprint

DNA
Information Storage and Retrieval
Durability
Genes
INDEL Mutation
Genome
Information Theory
Data storage equipment
Sequence Alignment
Information theory
Technology
Experiments

Keywords

  • DNa data storage
  • Error check
  • Error correction
  • Genetically modified organism (GMO)
  • Polymerase chain reaction (PCR)
  • Sequence alignment

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Biology

Cite this

Stabilizing synthetic data in the DNA of living organisms. / Yachie, Nozomu; Ohashi, Yoshiaki; Tomita, Masaru.

In: Systems and Synthetic Biology, Vol. 2, No. 1-2, 06.2008, p. 19-25.

Research output: Contribution to journalArticle

Yachie, Nozomu ; Ohashi, Yoshiaki ; Tomita, Masaru. / Stabilizing synthetic data in the DNA of living organisms. In: Systems and Synthetic Biology. 2008 ; Vol. 2, No. 1-2. pp. 19-25.
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