Abstract
Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both systems are affected by the Bsu restriction-modification system that recognizes and cleaves unmethylated XhoI sites, limiting the choice of target DNA. We constructed B. subtilis 168 with stable mutation for restriction-deficient and modification-proficient (r-m+). It was demonstrated that the r-m+ strains can incorporate and transfer synthesized DNA with multiple XhoI sites. These should be of value as hub strains to integrate and disseminate giant DNA between B. subtilis 168 derivatives.
| Original language | English |
|---|---|
| Pages (from-to) | 231-236 |
| Number of pages | 6 |
| Journal | Journal of biochemistry |
| Volume | 166 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2019 Sep 1 |
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Keywords
- Bacillus subtilis
- conjugation transfer
- DNA synthesis
- genome engineering
- natural genetic transformation
- restriction–modification
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168 : hub strains for giant DNA engineering. / Itaya, Mitsuhiro; Sato, Mitsuru; Watanabe, Satoru; Yoshikawa, Hirofumi; Tomita, Masaru; Sato, Rintaro.
In: Journal of biochemistry, Vol. 166, No. 3, 01.09.2019, p. 231-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168
T2 - hub strains for giant DNA engineering
AU - Itaya, Mitsuhiro
AU - Sato, Mitsuru
AU - Watanabe, Satoru
AU - Yoshikawa, Hirofumi
AU - Tomita, Masaru
AU - Sato, Rintaro
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both systems are affected by the Bsu restriction-modification system that recognizes and cleaves unmethylated XhoI sites, limiting the choice of target DNA. We constructed B. subtilis 168 with stable mutation for restriction-deficient and modification-proficient (r-m+). It was demonstrated that the r-m+ strains can incorporate and transfer synthesized DNA with multiple XhoI sites. These should be of value as hub strains to integrate and disseminate giant DNA between B. subtilis 168 derivatives.
AB - Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both systems are affected by the Bsu restriction-modification system that recognizes and cleaves unmethylated XhoI sites, limiting the choice of target DNA. We constructed B. subtilis 168 with stable mutation for restriction-deficient and modification-proficient (r-m+). It was demonstrated that the r-m+ strains can incorporate and transfer synthesized DNA with multiple XhoI sites. These should be of value as hub strains to integrate and disseminate giant DNA between B. subtilis 168 derivatives.
KW - Bacillus subtilis
KW - conjugation transfer
KW - DNA synthesis
KW - genome engineering
KW - natural genetic transformation
KW - restriction–modification
UR - http://www.scopus.com/inward/record.url?scp=85071707183&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071707183&partnerID=8YFLogxK
U2 - 10.1093/jb/mvz031
DO - 10.1093/jb/mvz031
M3 - Article
C2 - 31004491
AN - SCOPUS:85071707183
VL - 166
SP - 231
EP - 236
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 3
ER -