Abstract
Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both systems are affected by the Bsu restriction-modification system that recognizes and cleaves unmethylated XhoI sites, limiting the choice of target DNA. We constructed B. subtilis 168 with stable mutation for restriction-deficient and modification-proficient (r-m+). It was demonstrated that the r-m+ strains can incorporate and transfer synthesized DNA with multiple XhoI sites. These should be of value as hub strains to integrate and disseminate giant DNA between B. subtilis 168 derivatives.
| Original language | English |
|---|---|
| Pages (from-to) | 231-236 |
| Number of pages | 6 |
| Journal | Journal of Deaf Studies and Deaf Education |
| Volume | 25 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2020 Jan 1 |
Keywords
- Bacillus subtilis
- DNA synthesis
- conjugation transfer
- genome engineering
- natural genetic transformation
- restriction-modification
ASJC Scopus subject areas
- Education
- Speech and Hearing
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Itaya, M., Sato, M., Watanabe, S., Yoshikawa, H., Tomita, M., & Sato, R. (2020). Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: Hub strains for giant DNA engineering. Journal of Deaf Studies and Deaf Education, 25(1), 231-236. https://doi.org/10.1093/jb/mvz031