Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements

Hiromi Kochiwa, Mitsuhiro Itaya, Masaru Tomita, Akio Kanai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ribonuclease H1 (RNase H1) is a widespread enzyme found in a range of organisms from viruses to humans. It is capable of degrading the RNA moiety of DNA-RNA hybrids and requires a bivalent ion for activity. In contrast with most eukaryotes, which have one gene encoding RNase H1, the activity of which depends on Mg2+ ions, Caenorhabditis elegans has four RNase H1-related genes, and one of them has an isoform produced by alternative splicing. However, little is known about the enzymatic features of the proteins encoded by these genes. To determine the differences between these enzymes, we compared the expression patterns of each RNase H1-related gene throughout the development of the nematode and the RNase H activities of their recombinant proteins. We found gene-specific expression patterns and different enzymatic features. In particular, besides the enzyme that displays the highest activity in the presence of Mg2+ ions, C. elegans has another enzyme that shows preference for Mn2+ ion as a cofactor. We characterized this Mn 2+-dependent RNase H1 for the first time in eukaryotes. These results suggest that there are at least two types of RNase H1 in C. elegans depending on the developmental stage of the organism.

Original languageEnglish
Pages (from-to)420-429
Number of pages10
JournalFEBS Journal
Volume273
Issue number2
DOIs
Publication statusPublished - 2006 Jan

Fingerprint

Caenorhabditis elegans
Substrate Specificity
Cations
Substrates
Enzymes
Genes
Ions
Eukaryota
RNA
Ribonuclease H
Gene encoding
Alternative Splicing
Viruses
Recombinant Proteins
ribonuclease HI
Protein Isoforms
Gene Expression
DNA
Proteins

Keywords

  • Alternative splicing
  • C. elegans
  • Development
  • Metal ion
  • RNase H1

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements. / Kochiwa, Hiromi; Itaya, Mitsuhiro; Tomita, Masaru; Kanai, Akio.

In: FEBS Journal, Vol. 273, No. 2, 01.2006, p. 420-429.

Research output: Contribution to journalArticle

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