Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats

Toshihiko Iizuka, Toshiyuki Tanaka, Makoto Suematsu, Soichiro Miura, Toshiki Watanabe, Ryuji Koike, Yuzuru Ishimura, Hiromasa Ishii, Nobuyuki Miyasaka, Masayuki Miyasaka

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Abstract

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is essential for lymphocyte trafficking to gut-associated lymphoid tissues and is implicated in inflammatory disorders in the gut and pancreatic islets. In this study, we examined the functional role of MAdCAM-1 during rat ontogeny using newly generated specific mAb. As previously observed in mice and humans, MAdCAM-1 was preferentially expressed in high endothelial venules (HEV) in gut- associated lymphoid tissues and venules of lamina propria in adult rats. Lymphocyte rolling and adhesion on HEV in Peyer's patches (PP) were completely abrogated with neutralizing anti-MAdCAM-1 mAb, in agreement with the notion that MAdCAM-1 is the principal HEV ligand for lymphocyte roiling and adhesion in adult PP. In the developing gastrointestinal tract, MAdCAM-1 was widely expressed in the venules of the lamina propria of fetal rats. In addition, MAdCAM-1 was also expressed in follicular dendritic cells in the neonatal PP. Interestingly, MAdCAM-1 expression was found also in nonmucosal tissues during ontogeny. MAdCAM-1 was transiently expressed in blood vascular endothelial cells in the fetal skin and neonatal thymus. Notably, MAdCAM-1- positive blood vessels were localized mainly in the cortico-medullary junction in the neonatal thymus and about 10-20% of thymocytes, most of which were either CD4, CD8 double positive or single positive specifically reacted with soluble MAdCAM-1 via integrin α4β7. After birth, MAdCAM-1 expression in thymus blood vessels disappeared and concomitantly, the soluble MAdCAM-1- reactive thymocytes were rapidly down-regulated. Our results suggest that MAdCAM-1 functions as a vascular addressin in not only mucosal, but also nonmucosal lymphoid tissues during ontogeny.

Original languageEnglish
Pages (from-to)2463-2471
Number of pages9
JournalJournal of Immunology
Volume164
Issue number5
Publication statusPublished - 2000 Mar 1

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Cell Adhesion Molecules
Embryonic Development
Venules
Peyer's Patches
Lymphoid Tissue
Thymus Gland
Lymphocytes
Thymocytes
Blood Vessels
Mucous Membrane
Follicular Dendritic Cells
Islets of Langerhans
Integrins
Gastrointestinal Tract
Endothelial Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Iizuka, T., Tanaka, T., Suematsu, M., Miura, S., Watanabe, T., Koike, R., ... Miyasaka, M. (2000). Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats. Journal of Immunology, 164(5), 2463-2471.

Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats. / Iizuka, Toshihiko; Tanaka, Toshiyuki; Suematsu, Makoto; Miura, Soichiro; Watanabe, Toshiki; Koike, Ryuji; Ishimura, Yuzuru; Ishii, Hiromasa; Miyasaka, Nobuyuki; Miyasaka, Masayuki.

In: Journal of Immunology, Vol. 164, No. 5, 01.03.2000, p. 2463-2471.

Research output: Contribution to journalArticle

Iizuka, T, Tanaka, T, Suematsu, M, Miura, S, Watanabe, T, Koike, R, Ishimura, Y, Ishii, H, Miyasaka, N & Miyasaka, M 2000, 'Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats', Journal of Immunology, vol. 164, no. 5, pp. 2463-2471.
Iizuka T, Tanaka T, Suematsu M, Miura S, Watanabe T, Koike R et al. Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats. Journal of Immunology. 2000 Mar 1;164(5):2463-2471.
Iizuka, Toshihiko ; Tanaka, Toshiyuki ; Suematsu, Makoto ; Miura, Soichiro ; Watanabe, Toshiki ; Koike, Ryuji ; Ishimura, Yuzuru ; Ishii, Hiromasa ; Miyasaka, Nobuyuki ; Miyasaka, Masayuki. / Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats. In: Journal of Immunology. 2000 ; Vol. 164, No. 5. pp. 2463-2471.
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