Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats

Toshihiko Iizuka, Toshiyuki Tanaka, Makoto Suematsu, Soichiro Miura, Toshiki Watanabe, Ryuji Koike, Yuzuru Ishimura, Hiromasa Ishii, Nobuyuki Miyasaka, Masayuki Miyasaka

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45 Citations (Scopus)

Abstract

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is essential for lymphocyte trafficking to gut-associated lymphoid tissues and is implicated in inflammatory disorders in the gut and pancreatic islets. In this study, we examined the functional role of MAdCAM-1 during rat ontogeny using newly generated specific mAb. As previously observed in mice and humans, MAdCAM-1 was preferentially expressed in high endothelial venules (HEV) in gut- associated lymphoid tissues and venules of lamina propria in adult rats. Lymphocyte rolling and adhesion on HEV in Peyer's patches (PP) were completely abrogated with neutralizing anti-MAdCAM-1 mAb, in agreement with the notion that MAdCAM-1 is the principal HEV ligand for lymphocyte roiling and adhesion in adult PP. In the developing gastrointestinal tract, MAdCAM-1 was widely expressed in the venules of the lamina propria of fetal rats. In addition, MAdCAM-1 was also expressed in follicular dendritic cells in the neonatal PP. Interestingly, MAdCAM-1 expression was found also in nonmucosal tissues during ontogeny. MAdCAM-1 was transiently expressed in blood vascular endothelial cells in the fetal skin and neonatal thymus. Notably, MAdCAM-1- positive blood vessels were localized mainly in the cortico-medullary junction in the neonatal thymus and about 10-20% of thymocytes, most of which were either CD4, CD8 double positive or single positive specifically reacted with soluble MAdCAM-1 via integrin α4β7. After birth, MAdCAM-1 expression in thymus blood vessels disappeared and concomitantly, the soluble MAdCAM-1- reactive thymocytes were rapidly down-regulated. Our results suggest that MAdCAM-1 functions as a vascular addressin in not only mucosal, but also nonmucosal lymphoid tissues during ontogeny.

Original languageEnglish
Pages (from-to)2463-2471
Number of pages9
JournalJournal of Immunology
Volume164
Issue number5
DOIs
Publication statusPublished - 2000 Mar 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Iizuka, T., Tanaka, T., Suematsu, M., Miura, S., Watanabe, T., Koike, R., Ishimura, Y., Ishii, H., Miyasaka, N., & Miyasaka, M. (2000). Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats. Journal of Immunology, 164(5), 2463-2471. https://doi.org/10.4049/jimmunol.164.5.2463