Stain-free resolution of unmyelinated axons in transgenic mice using fluorescence microscopy

Suresh Mohan, Ivan Coto Hernandez, Martin K. Selig, Shinsuke Shibata, Nate Jowett

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Though unmyelinated fibers predominate axon counts within peripheral nerves, they are frequently excluded in histomorphometric assessment as they cannot be readily resolved by light microscopy. Herein, we demonstrate stain-free resolution of unmyelinated axons in Sox10-Venus mice by widefield fluorescence imaging of sciatic nerve cryosections. Optional staining of cryosections using a rapid and nontoxic myelin-specific dye (FluoroMyelin Red) enables robust synchronous resolution of myelinated and unmyelinated fibers, comprising a high-throughput platform for neural histomorphometry.

Original languageEnglish
Pages (from-to)1178-1180
Number of pages3
JournalJournal of Neuropathology and Experimental Neurology
Volume78
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Keywords

  • Fluorescence
  • Microscopy
  • Myelinated
  • Nerve fibers
  • Peripheral nerve
  • Schwann cells
  • Unmyelinated

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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