@article{97f41016ba75491cbbdb99098533fba4,
title = "Stain-free resolution of unmyelinated axons in transgenic mice using fluorescence microscopy",
abstract = "Though unmyelinated fibers predominate axon counts within peripheral nerves, they are frequently excluded in histomorphometric assessment as they cannot be readily resolved by light microscopy. Herein, we demonstrate stain-free resolution of unmyelinated axons in Sox10-Venus mice by widefield fluorescence imaging of sciatic nerve cryosections. Optional staining of cryosections using a rapid and nontoxic myelin-specific dye (FluoroMyelin Red) enables robust synchronous resolution of myelinated and unmyelinated fibers, comprising a high-throughput platform for neural histomorphometry.",
keywords = "Fluorescence, Microscopy, Myelinated, Nerve fibers, Peripheral nerve, Schwann cells, Unmyelinated",
author = "Suresh Mohan and Hernandez, {Ivan Coto} and Selig, {Martin K.} and Shinsuke Shibata and Nate Jowett",
note = "Funding Information: Send correspondence to: Suresh Mohan, MD, Surgical Photonics and Engi-neering Laboratory, Department of Otolaryngology – Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, 243 Charles Street, Boston, MA 02114; E-mail: suresh_mohan@meei. harvard.edu This study was supported by NIDCD NIH T32DC000020 (S.M.), a grant from Brain/MINDS by AMED #JP18dm0207002 (S.S.), and a generous donation from the Berthiaume Family Foundation, Inc (N.J.). The con-tent is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: This study was supported by NIDCD NIH T32DC000020 (S.M.), a grant from Brain/MINDS by AMED #JP18dm0207002 (S.S.), and a generous donation from the Berthiaume Family Foundation, Inc (N.J.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2019 American Association of Neuropathologists, Inc. All rights reserved.",
year = "2019",
month = dec,
day = "1",
doi = "10.1093/jnen/nlz099",
language = "English",
volume = "78",
pages = "1178--1180",
journal = "Journal of Neuropathology and Experimental Neurology",
issn = "0022-3069",
number = "12",
}