STAP-2 protein expression in B16F10 melanoma cells positively regulates protein levels of tyrosinase, which determines organs to infiltrate in the body

Yuichi Sekine, Sumihito Togi, Ryuta Muromoto, Shigeyuki Kon, Yuichi Kitai, Akihiko Yoshimura, Kenji Oritani, Tadashi Matsuda

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2 Citations (Scopus)

Abstract

Melanoma is the most serious type of skin cancer, with a highly metastatic phenotype. In this report, we show that signal transducing adaptor protein 2 (STAP-2) is involved in cell migration, proliferation, and melanogenesis as well as chemokine receptor expression and tumorigenesis in B16F10 melanoma cells. This was evident in mice injected with STAP-2 shRNA (shSTAP-2)-expressing B16F10 cells, which infiltrated organs in a completely different pattern from the original cells, showing massive colonization in the liver, kidney, and neck but not in the lung. The most important finding was that STAP-2 expression determined tyrosinase protein content. STAP-2 colocalized with tyrosinase in lysosomes and protected tyrosinase from protein degradation. It is noteworthy that B16F10 cells with knocked down tyrosinase showed similar cell characteristics as shSTAP-2 cells. These results indicated that tyrosinase contributed to some cellular events beyond melanogenesis. Taken together, one possibility is that STAP-2 positively regulates the protein levels of tyrosinase, which determines tumor invasion via controlling chemokine receptor expression.

Original languageEnglish
Pages (from-to)17462-17473
Number of pages12
JournalJournal of Biological Chemistry
Volume290
Issue number28
DOIs
Publication statusPublished - 2015 Jul 10

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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