STAP-2 protein promotes prostate cancer growth by enhancing epidermal growth factor receptor stabilization

Yuichi Kitai, Masashi Iwakami, Kodai Saitoh, Sumihito Togi, Serina Isayama, Yuichi Sekine, Ryuta Muromoto, Jun Ichi Kashiwakura, Akihiko Yoshimura, Kenji Oritani, Tadashi Matsuda

Research output: Contribution to journalArticle

Abstract

Signal-transducing adaptor family member-2 (STAP-2) is an adaptor protein that regulates various intracellular signaling pathways and promotes tumorigenesis in melanoma and breast cancer cells. However, the contribution of STAP-2 to the behavior of other types of cancer cells is unclear. Here, we show that STAP-2 promotes tumorigenesis of prostate cancer cells through up-regulation of EGF receptor (EGFR) signaling. Tumor growth of a prostate cancer cell line, DU145, was strongly decreased by STAP-2 knockdown. EGF-induced gene expression and phosphorylation of AKT, ERK, and STAT3 were significantly decreased in STAP-2–knockdown DU145 cells. Mechanistically, we found that STAP-2 interacted with EGFR and enhanced its stability by inhibiting c-CBL–mediated EGFR ubiquitination. Our results indicate that STAP-2 promotes prostate cancer progression via facilitating EGFR activation.

Original languageEnglish
Pages (from-to)19392-19399
Number of pages8
JournalJournal of Biological Chemistry
Volume292
Issue number47
DOIs
Publication statusPublished - 2017 Jan 1

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Epidermal Growth Factor Receptor
Prostatic Neoplasms
Stabilization
Cells
Growth
Proteins
Space time adaptive processing
Phosphorylation
Carcinogenesis
Epidermal Growth Factor
Gene expression
Tumors
Ubiquitination
Chemical activation
Melanoma
Neoplasms
Up-Regulation
Breast Neoplasms
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kitai, Y., Iwakami, M., Saitoh, K., Togi, S., Isayama, S., Sekine, Y., ... Matsuda, T. (2017). STAP-2 protein promotes prostate cancer growth by enhancing epidermal growth factor receptor stabilization. Journal of Biological Chemistry, 292(47), 19392-19399. https://doi.org/10.1074/jbc.M117.802884

STAP-2 protein promotes prostate cancer growth by enhancing epidermal growth factor receptor stabilization. / Kitai, Yuichi; Iwakami, Masashi; Saitoh, Kodai; Togi, Sumihito; Isayama, Serina; Sekine, Yuichi; Muromoto, Ryuta; Kashiwakura, Jun Ichi; Yoshimura, Akihiko; Oritani, Kenji; Matsuda, Tadashi.

In: Journal of Biological Chemistry, Vol. 292, No. 47, 01.01.2017, p. 19392-19399.

Research output: Contribution to journalArticle

Kitai, Y, Iwakami, M, Saitoh, K, Togi, S, Isayama, S, Sekine, Y, Muromoto, R, Kashiwakura, JI, Yoshimura, A, Oritani, K & Matsuda, T 2017, 'STAP-2 protein promotes prostate cancer growth by enhancing epidermal growth factor receptor stabilization', Journal of Biological Chemistry, vol. 292, no. 47, pp. 19392-19399. https://doi.org/10.1074/jbc.M117.802884
Kitai, Yuichi ; Iwakami, Masashi ; Saitoh, Kodai ; Togi, Sumihito ; Isayama, Serina ; Sekine, Yuichi ; Muromoto, Ryuta ; Kashiwakura, Jun Ichi ; Yoshimura, Akihiko ; Oritani, Kenji ; Matsuda, Tadashi. / STAP-2 protein promotes prostate cancer growth by enhancing epidermal growth factor receptor stabilization. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 47. pp. 19392-19399.
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