STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells

Osamu Ikeda, Yuichi Sekine, Michinori Kakisaka, Satoshi Tsuji, Ryuta Muromoto, Norihiko Ohbayashi, Kenji Oritani, Akihiko Yoshimura, Tadashi Matsuda

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Abstract

Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. Our previous studies also revealed that STAP-2 binds to MyD88 and IKK-α/β, and modulates NF-κB signaling in macrophages. In the present study, we examined physiological roles of the interaction between STAP-2 and c-Fms in Raw 264.7 macrophage cells. Our immunoprecipitation has revealed that c-Fms directly interacts with the PH domain of STAP-2 independently on M-CSF-stimulation. Ectopic expression of STAP-2 markedly suppressed M-CSF-induced tyrosine phosphorylation of c-Fms as well as activation of Akt and extracellular signal regulated kinase. In addition, Raw 264.7 cells over-expressing STAP-2 showed impaired migration in response to M-CSF and wound-healing process. Taken together, our findings demonstrate that STAP-2 directly binds to c-Fms and interferes with the PI3K signaling, which leads to macrophage motility, in Raw 264.7 cells.

Original languageEnglish
Pages (from-to)931-937
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number3
DOIs
Publication statusPublished - 2007 Jul 6

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Keywords

  • Adaptor protein
  • Macrophage
  • Migration
  • Signal transduction
  • Tyrosine phosphorylation
  • c-Fms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ikeda, O., Sekine, Y., Kakisaka, M., Tsuji, S., Muromoto, R., Ohbayashi, N., Oritani, K., Yoshimura, A., & Matsuda, T. (2007). STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells. Biochemical and Biophysical Research Communications, 358(3), 931-937. https://doi.org/10.1016/j.bbrc.2007.05.030