Starfish ApDOCK protein essentially functions in larval defense system operated by mesenchyme cells

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5 Citations (Scopus)

Abstract

In larvae of the starfish, Asterina pectinifera, mesenchyme cells operate in the defense system through various behaviors. We have investigated mesenchyme cell dynamics during the immune response by identifying ApDOCK, a new member of the DOCK180 superfamily protein. In 4-day-old bipinnaria larvae processed for morpholino oligonucleotide-mediated knockdown of ApDOCK, injection of inorganic foreign substances revealed that (1) mesenchyme cells fail to undergo either directed migration toward a large oil-droplet or persistent spreading on the oil-droplet after contact; (2) neither uptake of micro-beads nor cell-to-cell fusion on the large oil-droplet differed from that of mesenchyme cells from control larvae. Similar behaviors were also recorded in experiments where bacteria were injected. Under culture conditions, the expression level of ApDOCK mRNA was significantly associated with the immunological behavior of mesenchyme cells. Apparently, the mesenchyme cells from ApDOCK loss-of-function larvae exhibited insufficient lamellipodium formation via lack of fibrous form of actin organization at the leading edge. These results suggest that the migratory congregation and persistence of encapsulation of larval mesenchyme cells are intracellularly regulated by ApDOCK protein, and this regulation is associated with organization of cytoskeletal actin.

Original languageEnglish
Pages (from-to)955-965
Number of pages11
JournalImmunology and Cell Biology
Volume90
Issue number10
DOIs
Publication statusPublished - 2012 Nov

Fingerprint

Starfish
Mesoderm
Larva
Proteins
Oils
Actins
Asterina
Morpholinos
Pseudopodia
Cell Fusion
Bacteria
Messenger RNA
Injections

Keywords

  • Cell migration
  • DOCK180 superfamily
  • Encapsulation
  • Lamellipodium formation
  • Larval mesenchyme cells
  • Starfish

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

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title = "Starfish ApDOCK protein essentially functions in larval defense system operated by mesenchyme cells",
abstract = "In larvae of the starfish, Asterina pectinifera, mesenchyme cells operate in the defense system through various behaviors. We have investigated mesenchyme cell dynamics during the immune response by identifying ApDOCK, a new member of the DOCK180 superfamily protein. In 4-day-old bipinnaria larvae processed for morpholino oligonucleotide-mediated knockdown of ApDOCK, injection of inorganic foreign substances revealed that (1) mesenchyme cells fail to undergo either directed migration toward a large oil-droplet or persistent spreading on the oil-droplet after contact; (2) neither uptake of micro-beads nor cell-to-cell fusion on the large oil-droplet differed from that of mesenchyme cells from control larvae. Similar behaviors were also recorded in experiments where bacteria were injected. Under culture conditions, the expression level of ApDOCK mRNA was significantly associated with the immunological behavior of mesenchyme cells. Apparently, the mesenchyme cells from ApDOCK loss-of-function larvae exhibited insufficient lamellipodium formation via lack of fibrous form of actin organization at the leading edge. These results suggest that the migratory congregation and persistence of encapsulation of larval mesenchyme cells are intracellularly regulated by ApDOCK protein, and this regulation is associated with organization of cytoskeletal actin.",
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author = "Ryohei Furukawa and Hiromi Funabashi and Midori Matsumoto and Hiroyuki Kaneko",
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AU - Funabashi, Hiromi

AU - Matsumoto, Midori

AU - Kaneko, Hiroyuki

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