@article{2e198d6ce77e40188dd699271b6fc9c9,
title = "Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes)",
abstract = "The teleost fish, medaka (Oryzias latipes), employs the XX/XY genetic sex determination system. We show here that the phenotypic sex of medaka is affected by changes in lipid metabolism. Medaka larvae subjected to 5 days of starvation underwent female-to-male sex reversal. Metabolomic and RT-qPCR analyses indicated that pantothenate metabolism was suppressed by starvation. Consistently, inhibiting the pantothenate metabolic pathway caused sex reversal. The final metabolite in this pathway is coenzyme A, an essential factor for lipogenesis. Inhibiting fatty acid synthesis, the first step of lipogenesis, also caused sex reversal. The expression of dmrt1, a critical gene for male development, was suppressed by starvation, and a dmrt1 (Δ13) mutant did not show sex reversal under starvation. Collectively, these results indicate that fatty acid synthesis is involved in female-to-male sex reversal through ectopic expression of male gene dmrt1 under starvation.",
keywords = "Fatty acid synthesis, Metabolome, Pantothenate metabolism, Sex reversal",
author = "Yuta Sakae and Akira Oikawa and Yuki Sugiura and Masatoshi Mita and Shuhei Nakamura and Toshiya Nishimura and Makoto Suematsu and Minoru Tanaka",
note = "Funding Information: This work was partly supported by Research Fellowships of Japan Society for the Promotion of Science for Young Scientists [15J07867, Y.S.], Grant-in-Aid for Scientific Research on Innovative Areas [17H06430, M.T.], a Grant-in-Aid for Scientific Research (A) [16H02514, M.T.] and the NARO Bio-oriented Technology Research Advancement Institution [Research Program on Development of Innovative Technology, 26047AB, M.T.]. Funding Information: We thank the members of the Tanaka lab at Nagoya University and previous lab members at NIBB for informative suggestions, as well as Ms I. Watakabe, Ms C. Kinoshita, and Mr K. Suzuki for fish maintenance. We appreciate NBRP Medaka for providing the d-rR strain. This work was partly supported by Research Fellowships of Japan Society for the Promotion of Science for Young Scientists [15J07867, Y.S.], Grant-in-Aid for Scientific Research on Innovative Areas [17H06430, M.T.], a Grant-in-Aid for Scientific Research (A) [16H02514, M.T.] and the NARO Bio-oriented Technology Research Advancement Institution [Research Program on Development of Innovative Technology, 26047AB, M.T.]. Publisher Copyright: {\textcopyright} 2020 Company of Biologists Ltd. All rights reserved.",
year = "2020",
month = apr,
doi = "10.1242/bio.050054",
language = "English",
volume = "9",
journal = "Biology Open",
issn = "2046-6390",
publisher = "Company of Biologists Ltd",
number = "4",
}