Static and dynamic mechanical properties of extracellular matrix synthesized by cultured chondrocytes

Shogo Miyata, Katsuko S. Furukawa, Takashi Ushida, Yasuo Nitta, Tetsuya Tateishi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recently, an approach to restore cartilage defects, culturing autologous chondrocytes in vitro to create a three-dimensional tissue and then implanting the cultured tissue, has been developed. In this kind of approach, it is important to study whether the extracellular matrixes synthesized by cultured chondrocytes have appropriate mechanical property. In this study, we attempted to evaluate in detail mechanical properties of the extracellular matrix synthesized by chondrocytes in vitro. Therefore, we cultured bovine chondrocytes in agarose gel and assessed the changes in not only static but also dynamic mechanical properties during long-term culture. In addition, to assess the mechanical property of the extracellular matrix synthesized by chondrocytes only, we calculated the volume ratio of the extracellular matrix in agarose gel from the cross section and estimated the static Young's modulus of the extracellular matrix synthesized by cultured chondrocytes based on the Rule of Mixture. As a result, both the equilibrium aggregate modulus and dynamic Young's modulus of chondrocyte/agarose disks increased with time. The value of calculated modulus was larger than that of the pericellular matrix of cartilage explant. In conclusion, the biomechanical property of chondrocyte/agarose constructs and the matrix synthesized by chondrocytes were assessed in detail.

Original languageEnglish
Pages (from-to)425-429
Number of pages5
JournalMaterials Science and Engineering C
Volume24
Issue number3
DOIs
Publication statusPublished - 2004 Apr 1
Externally publishedYes

Fingerprint

Sepharose
mechanical properties
Mechanical properties
Cartilage
matrices
Gels
Elastic moduli
Tissue
cartilage
modulus of elasticity
gels
Defects
cross sections
defects

Keywords

  • Chondrocyte
  • Confined compression test
  • Dynamic visco-elasticity
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials

Cite this

Static and dynamic mechanical properties of extracellular matrix synthesized by cultured chondrocytes. / Miyata, Shogo; Furukawa, Katsuko S.; Ushida, Takashi; Nitta, Yasuo; Tateishi, Tetsuya.

In: Materials Science and Engineering C, Vol. 24, No. 3, 01.04.2004, p. 425-429.

Research output: Contribution to journalArticle

Miyata, Shogo ; Furukawa, Katsuko S. ; Ushida, Takashi ; Nitta, Yasuo ; Tateishi, Tetsuya. / Static and dynamic mechanical properties of extracellular matrix synthesized by cultured chondrocytes. In: Materials Science and Engineering C. 2004 ; Vol. 24, No. 3. pp. 425-429.
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