Staurosporine, a potent protein kinase C inhibitor, fails to inhibit 12-O-tetradecanoylphorbol-13-acetate-caused ornithine decarboxylase induction in isolated mouse epidermal cells

Itsumi Kiyoto, Satoshi Yamamoto, Eriko Yokota, Ryuichi Kato

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Staurosporine, a most potent protein kinase C inhibitor, actually inhibited protein kinase C activity obtained either from cytosol or particulate fraction of mouse epidermis. Staurosporine at the concentrations which exert protein kinase C inhibition, however, failed to inhibit, but markedly augmented 12-O-tetradecanoylphorbol-13-acetate (TPA)-caused ornithine decarboxylase (ODC) induction in isolated mouse epidermal cells. Staurosporine by itself induced ODC activity as TPA does. Mechanism of ODC induction seems different between these two compounds. Another protein kinase C inhibitor, H-7, inhibited both staurosporine- and TPA-caused ODC induction.

Original languageEnglish
Pages (from-to)740-746
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number2
DOIs
Publication statusPublished - 1987 Oct 29

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Ornithine Decarboxylase
Staurosporine
Protein C Inhibitor
Tetradecanoylphorbol Acetate
Protein Kinase Inhibitors
Protein Kinase C
Acetates
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Epidermis
Cytosol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "Staurosporine, a most potent protein kinase C inhibitor, actually inhibited protein kinase C activity obtained either from cytosol or particulate fraction of mouse epidermis. Staurosporine at the concentrations which exert protein kinase C inhibition, however, failed to inhibit, but markedly augmented 12-O-tetradecanoylphorbol-13-acetate (TPA)-caused ornithine decarboxylase (ODC) induction in isolated mouse epidermal cells. Staurosporine by itself induced ODC activity as TPA does. Mechanism of ODC induction seems different between these two compounds. Another protein kinase C inhibitor, H-7, inhibited both staurosporine- and TPA-caused ODC induction.",
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AU - Yokota, Eriko

AU - Kato, Ryuichi

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