Steady and pulsatile shear stress induce different three-dimensional endothelial networks through pseudopodium formation

Yoshinori Abe, Ryo Sudo, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

Abstract

Control of angiogenesis is a major challenge to promotion of vascularization in the field of tissue engineering. In particular, shear stress is recognized as an important mechanical factor controlling new vessel formation. However, the effects of steady and pulsatile shear stress on endothelial cell (EC) network formation remain unclear. Here, we systematically investigated their effects. Compared with pulsatile shear stress, steady shear stress at 1.0 Pa increased cell numbers in EC networks as well as the distribution of networks and pseudopodia in the deep range after 48 h. To further investigate the process of EC network growth, we focused on the effect of flow frequency on network elongation dynamics. Pulsatile shear stress at 1.0 Pa increased the extension and retraction velocities and separation of networks, resulting in the formation of unstable EC networks. In contrast, steady shear stress application resulted in the formation of extended and stable EC networks composed of many cells. Thus, two types of three-dimensional network growth were observed, depending on flow pulsatility. A combination of the type of ECs, such as aortic and microvascular ECs, and flow characteristics, such as flow magnitude and frequency, may have important implications for the construction of well-developed three-dimensional EC networks.

Original languageEnglish
Pages (from-to)38-48
Number of pages11
JournalJournal of Biorheology
Volume27
Issue number1-2
DOIs
Publication statusPublished - 2013 Nov

Fingerprint

Endothelial cells
Shear stress
Tissue engineering
Elongation

Keywords

  • Angiogenesis
  • Endothelial cell
  • Fluid shear stress
  • Pseudopodium
  • Three-dimensional network

ASJC Scopus subject areas

  • Mechanics of Materials
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Steady and pulsatile shear stress induce different three-dimensional endothelial networks through pseudopodium formation. / Abe, Yoshinori; Sudo, Ryo; Ikeda, Mariko; Tanishita, Kazuo.

In: Journal of Biorheology, Vol. 27, No. 1-2, 11.2013, p. 38-48.

Research output: Contribution to journalArticle

@article{504460a3e389468c965610bfe100f95f,
title = "Steady and pulsatile shear stress induce different three-dimensional endothelial networks through pseudopodium formation",
abstract = "Control of angiogenesis is a major challenge to promotion of vascularization in the field of tissue engineering. In particular, shear stress is recognized as an important mechanical factor controlling new vessel formation. However, the effects of steady and pulsatile shear stress on endothelial cell (EC) network formation remain unclear. Here, we systematically investigated their effects. Compared with pulsatile shear stress, steady shear stress at 1.0 Pa increased cell numbers in EC networks as well as the distribution of networks and pseudopodia in the deep range after 48 h. To further investigate the process of EC network growth, we focused on the effect of flow frequency on network elongation dynamics. Pulsatile shear stress at 1.0 Pa increased the extension and retraction velocities and separation of networks, resulting in the formation of unstable EC networks. In contrast, steady shear stress application resulted in the formation of extended and stable EC networks composed of many cells. Thus, two types of three-dimensional network growth were observed, depending on flow pulsatility. A combination of the type of ECs, such as aortic and microvascular ECs, and flow characteristics, such as flow magnitude and frequency, may have important implications for the construction of well-developed three-dimensional EC networks.",
keywords = "Angiogenesis, Endothelial cell, Fluid shear stress, Pseudopodium, Three-dimensional network",
author = "Yoshinori Abe and Ryo Sudo and Mariko Ikeda and Kazuo Tanishita",
year = "2013",
month = "11",
doi = "10.1007/s12573-012-0056-5",
language = "English",
volume = "27",
pages = "38--48",
journal = "Journal of Biorheology",
issn = "1867-0466",
publisher = "Springer Japan",
number = "1-2",

}

TY - JOUR

T1 - Steady and pulsatile shear stress induce different three-dimensional endothelial networks through pseudopodium formation

AU - Abe, Yoshinori

AU - Sudo, Ryo

AU - Ikeda, Mariko

AU - Tanishita, Kazuo

PY - 2013/11

Y1 - 2013/11

N2 - Control of angiogenesis is a major challenge to promotion of vascularization in the field of tissue engineering. In particular, shear stress is recognized as an important mechanical factor controlling new vessel formation. However, the effects of steady and pulsatile shear stress on endothelial cell (EC) network formation remain unclear. Here, we systematically investigated their effects. Compared with pulsatile shear stress, steady shear stress at 1.0 Pa increased cell numbers in EC networks as well as the distribution of networks and pseudopodia in the deep range after 48 h. To further investigate the process of EC network growth, we focused on the effect of flow frequency on network elongation dynamics. Pulsatile shear stress at 1.0 Pa increased the extension and retraction velocities and separation of networks, resulting in the formation of unstable EC networks. In contrast, steady shear stress application resulted in the formation of extended and stable EC networks composed of many cells. Thus, two types of three-dimensional network growth were observed, depending on flow pulsatility. A combination of the type of ECs, such as aortic and microvascular ECs, and flow characteristics, such as flow magnitude and frequency, may have important implications for the construction of well-developed three-dimensional EC networks.

AB - Control of angiogenesis is a major challenge to promotion of vascularization in the field of tissue engineering. In particular, shear stress is recognized as an important mechanical factor controlling new vessel formation. However, the effects of steady and pulsatile shear stress on endothelial cell (EC) network formation remain unclear. Here, we systematically investigated their effects. Compared with pulsatile shear stress, steady shear stress at 1.0 Pa increased cell numbers in EC networks as well as the distribution of networks and pseudopodia in the deep range after 48 h. To further investigate the process of EC network growth, we focused on the effect of flow frequency on network elongation dynamics. Pulsatile shear stress at 1.0 Pa increased the extension and retraction velocities and separation of networks, resulting in the formation of unstable EC networks. In contrast, steady shear stress application resulted in the formation of extended and stable EC networks composed of many cells. Thus, two types of three-dimensional network growth were observed, depending on flow pulsatility. A combination of the type of ECs, such as aortic and microvascular ECs, and flow characteristics, such as flow magnitude and frequency, may have important implications for the construction of well-developed three-dimensional EC networks.

KW - Angiogenesis

KW - Endothelial cell

KW - Fluid shear stress

KW - Pseudopodium

KW - Three-dimensional network

UR - http://www.scopus.com/inward/record.url?scp=84888129416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888129416&partnerID=8YFLogxK

U2 - 10.1007/s12573-012-0056-5

DO - 10.1007/s12573-012-0056-5

M3 - Article

AN - SCOPUS:84888129416

VL - 27

SP - 38

EP - 48

JO - Journal of Biorheology

JF - Journal of Biorheology

SN - 1867-0466

IS - 1-2

ER -