Stem cell self-renewal factors Bmi1 and HMGA2 in head and neck squamous cell carcinoma

Clues for diagnosis

Hiroshi Yamazaki, Taisuke Mori, Masaki Yazawa, Akiko M. Maeshima, Fumihiko Matsumoto, Seiichi Yoshimoto, Yoshihide Ota, Akihiro Kaneko, Hitoshi Tsuda, Yae Kanai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Head and neck squamous cell carcinoma (HNSCC) includes both morphological and functional cellular heterogeneity, as would be expected if it arose from dysregulated stem or progenitor cells as opposed to the simple clonal expansion of a mutated cell; however, stemness molecule expression levels and distribution in HNSCC remain unclear. To clarify this, stemness molecule expressions were determined in HNSCC, as well as their properties and prognosis. Two proto-oncogenic chromatin regulators, Bmi-1 and high-mobility-group A2 (Hmga2), were identified in 12 pair cases of HNSCC tumor regions by comparison with their non-cancerous background tissues using cDNA microarray. Both Bmi-1 and Hmga2 are known to promote stem cell self-renewal by negatively regulating the expressions of Ink4a and Arf tumor suppressors. Despite similar targets, Bmi-1 protein was expressed in an early cancerous region and HMGA2 protein was expressed in a region showing more progression. Similarly, Bmi1 expression had no significance with regard to overall survival (P=0.67), whereas HMGA2 expression was associated with decreased overall survival (P=0.05). Quantitative real-time reverse transcription polymerase chain reaction analyses also correlated with protein levels. These findings suggest that Bmi-1 is an early detection marker to distinguish cancerous from non-cancerous regions, whereas HMGA2 is presumed to be a tumor prognosis marker. Among our HNSCC analyses, these stemness molecules expressed fewer primitive rare cells in the tumor than all other cells in the tumor. HNSCC cells with high expression of stemness molecules partly behave like stem cells.

Original languageEnglish
Pages (from-to)1331-1338
Number of pages8
JournalLaboratory Investigation
Volume93
Issue number12
DOIs
Publication statusPublished - 2013

Fingerprint

Stem Cells
varespladib methyl
HMGA2 Protein
Neoplasms
Tumor Biomarkers
Oligonucleotide Array Sequence Analysis
Reverse Transcription
Chromatin
Carcinoma, squamous cell of head and neck
Cell Self Renewal
Proteins
Polymerase Chain Reaction

Keywords

  • Bmi1
  • head and neck
  • HMGA2
  • squamous cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Stem cell self-renewal factors Bmi1 and HMGA2 in head and neck squamous cell carcinoma : Clues for diagnosis. / Yamazaki, Hiroshi; Mori, Taisuke; Yazawa, Masaki; Maeshima, Akiko M.; Matsumoto, Fumihiko; Yoshimoto, Seiichi; Ota, Yoshihide; Kaneko, Akihiro; Tsuda, Hitoshi; Kanai, Yae.

In: Laboratory Investigation, Vol. 93, No. 12, 2013, p. 1331-1338.

Research output: Contribution to journalArticle

Yamazaki, H, Mori, T, Yazawa, M, Maeshima, AM, Matsumoto, F, Yoshimoto, S, Ota, Y, Kaneko, A, Tsuda, H & Kanai, Y 2013, 'Stem cell self-renewal factors Bmi1 and HMGA2 in head and neck squamous cell carcinoma: Clues for diagnosis', Laboratory Investigation, vol. 93, no. 12, pp. 1331-1338. https://doi.org/10.1038/labinvest.2013.120
Yamazaki, Hiroshi ; Mori, Taisuke ; Yazawa, Masaki ; Maeshima, Akiko M. ; Matsumoto, Fumihiko ; Yoshimoto, Seiichi ; Ota, Yoshihide ; Kaneko, Akihiro ; Tsuda, Hitoshi ; Kanai, Yae. / Stem cell self-renewal factors Bmi1 and HMGA2 in head and neck squamous cell carcinoma : Clues for diagnosis. In: Laboratory Investigation. 2013 ; Vol. 93, No. 12. pp. 1331-1338.
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