Stem cell separation using thermoresponsive copolymer brushes having cationic charge

Kenichi Nagase, Yuri Hatakeyama, Tatsuya Shimizu, Katsuhisa Matsuura, Masayuki Yamato, Naoya Takeda, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

For developing new stem cell separation tool, we have prepared thermoresponsive cationic copolymer brush, poly(N-isopropylacrylamide-co-N, N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide (IPAAm-co-DMAPAAm-co-tBAAm) on glass substrates through surface-initiated atom transfer radical copolymerization (ATRP). Using the prepared thermoresponsive cationic copolymer brush, mesenchymal stem cell separation was performed simply by changing temperature.

Original languageEnglish
Title of host publication2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479966783
DOIs
Publication statusPublished - 2016 Mar 21
Externally publishedYes
EventInternational Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 - Nagoya, Japan
Duration: 2015 Nov 232015 Nov 25

Other

OtherInternational Symposium on Micro-NanoMechatronics and Human Science, MHS 2015
CountryJapan
CityNagoya
Period15/11/2315/11/25

Fingerprint

Cell Separation
Brushes
Stem cells
Stem Cells
Copolymers
Mesenchymal Stromal Cells
Copolymerization
Glass
Atoms
Temperature
Substrates
tert-butylacrylamide
N,N-dimethylaminopropylacrylamide
poly-N-isopropylacrylamide

ASJC Scopus subject areas

  • Biomedical Engineering
  • Electrical and Electronic Engineering
  • Biotechnology
  • Education

Cite this

Nagase, K., Hatakeyama, Y., Shimizu, T., Matsuura, K., Yamato, M., Takeda, N., & Okano, T. (2016). Stem cell separation using thermoresponsive copolymer brushes having cationic charge. In 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 [7438250] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2015.7438250

Stem cell separation using thermoresponsive copolymer brushes having cationic charge. / Nagase, Kenichi; Hatakeyama, Yuri; Shimizu, Tatsuya; Matsuura, Katsuhisa; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015. Institute of Electrical and Electronics Engineers Inc., 2016. 7438250.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nagase, K, Hatakeyama, Y, Shimizu, T, Matsuura, K, Yamato, M, Takeda, N & Okano, T 2016, Stem cell separation using thermoresponsive copolymer brushes having cationic charge. in 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015., 7438250, Institute of Electrical and Electronics Engineers Inc., International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015, Nagoya, Japan, 15/11/23. https://doi.org/10.1109/MHS.2015.7438250
Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N et al. Stem cell separation using thermoresponsive copolymer brushes having cationic charge. In 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015. Institute of Electrical and Electronics Engineers Inc. 2016. 7438250 https://doi.org/10.1109/MHS.2015.7438250
Nagase, Kenichi ; Hatakeyama, Yuri ; Shimizu, Tatsuya ; Matsuura, Katsuhisa ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Stem cell separation using thermoresponsive copolymer brushes having cationic charge. 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015. Institute of Electrical and Electronics Engineers Inc., 2016.
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