Abstract
Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.
| Original language | English |
|---|---|
| Pages (from-to) | 91-96 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 372 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Jul 18 |
| Externally published | Yes |
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Keywords
- Dax1/Nr0b1/Ahch
- ES cells
- Nuclear hormone receptor
- Oct3/4
- Self-renewal
- Sox2
- STAT3
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells. / Sun, Chuanhai; Nakatake, Yuki; Ura, Hiroki; Akagi, Tadayuki; Niwa, Hitoshi; Koide, Hiroshi; Yokota, Takashi.
In: Biochemical and Biophysical Research Communications, Vol. 372, No. 1, 18.07.2008, p. 91-96.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells
AU - Sun, Chuanhai
AU - Nakatake, Yuki
AU - Ura, Hiroki
AU - Akagi, Tadayuki
AU - Niwa, Hitoshi
AU - Koide, Hiroshi
AU - Yokota, Takashi
PY - 2008/7/18
Y1 - 2008/7/18
N2 - Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.
AB - Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.
KW - Dax1/Nr0b1/Ahch
KW - ES cells
KW - Nuclear hormone receptor
KW - Oct3/4
KW - Self-renewal
KW - Sox2
KW - STAT3
UR - http://www.scopus.com/inward/record.url?scp=44649183963&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44649183963&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.04.154
DO - 10.1016/j.bbrc.2008.04.154
M3 - Article
C2 - 18471437
AN - SCOPUS:44649183963
VL - 372
SP - 91
EP - 96
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -