Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells

Chuanhai Sun; Yuhki Nakatake; Hiroki Ura; Tadayuki Akagi; Hitoshi Niwa; Hiroshi Koide; Takashi Yokota

doi:10.1016/j.bbrc.2008.04.154

Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells

Chuanhai Sun, Yuhki Nakatake, Hiroki Ura, Tadayuki Akagi, Hitoshi Niwa, Hiroshi Koide, Takashi Yokota

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.

Original language	English
Pages (from-to)	91-96
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.04.154
Publication status	Published - 2008 Jul 18
Externally published	Yes

Keywords

Dax1/Nr0b1/Ahch
ES cells
Nuclear hormone receptor
Oct3/4
STAT3
Self-renewal
Sox2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells. / Sun, Chuanhai; Nakatake, Yuhki; Ura, Hiroki; Akagi, Tadayuki; Niwa, Hitoshi; Koide, Hiroshi; Yokota, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 372, No. 1, 18.07.2008, p. 91-96.

Research output: Contribution to journal › Article › peer-review

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title = "Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells",

abstract = "Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.",

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author = "Chuanhai Sun and Yuhki Nakatake and Hiroki Ura and Tadayuki Akagi and Hitoshi Niwa and Hiroshi Koide and Takashi Yokota",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. ",

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AU - Sun, Chuanhai

AU - Nakatake, Yuhki

AU - Ura, Hiroki

AU - Akagi, Tadayuki

AU - Niwa, Hitoshi

AU - Koide, Hiroshi

AU - Yokota, Takashi

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N2 - Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.

AB - Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.

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Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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