Stem cell-specific expression of Dax1 is conferred by STAT3 and Oct3/4 in embryonic stem cells

Chuanhai Sun, Yuhki Nakatake, Hiroki Ura, Tadayuki Akagi, Hitoshi Niwa, Hiroshi Koide, Takashi Yokota

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are pluripotent cells derived from inner cell mass of blastocysts. An orphan nuclear receptor, Dax1, is specifically expressed in undifferentiated ES cells and plays an important role in their self-renewal. The regulatory mechanism of Dax1 expression in ES cells, however, remains unknown. In this study, we found that STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. Suppression of either STAT3 or Oct3/4 resulted in down-regulation of Dax1. Reporter assay identified putative binding sites for these factors in the promoter/enhancer region of the Dax1 gene. Chromatin immunoprecipitation analysis suggested the in vivo association of STAT3 and Oct3/4 with the putative sites. Furthermore, gel shift assay indicated that these transcription factors directly bind to their putative binding sites. These results suggest that STAT3 and Oct3/4 control the expression of Dax1 to maintain the self-renewal of ES cells.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number1
DOIs
Publication statusPublished - 2008 Jul 18
Externally publishedYes

Keywords

  • Dax1/Nr0b1/Ahch
  • ES cells
  • Nuclear hormone receptor
  • Oct3/4
  • STAT3
  • Self-renewal
  • Sox2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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