Stem cells purified from human induced pluripotent stem cell-derived neural crest-like cells promote peripheral nerve regeneration

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Abstract

Strategies for therapeutic cell transplantation have been assessed for use in the treatment of massive peripheral nerve defects. To support safe and efficient cell transplantation, we have focused on the purification of cells using cell surface markers. Our group previously reported low-Affinity nerve growth factor receptor (LNGFR)-and thymocyte antigen-1 (THY-1)-positive neural crest-like cells (LT-NCLCs), generated from human induced pluripotent stem cells (hiPSCs). In the present study, we investigated the efficacy of transplantation of hiPSC-derived LT-NCLCs in a murine massive peripheral nerve defect model. Animals with a sciatic nerve defect were treated with a bridging silicone tube prefilled with LT-NCLCs or medium in the transplantation (TP) and negative control (NC) groups, respectively. The grafted LT-NCLCs survived and enhanced myelination and angiogenesis, as compared to the NC group. Behavioral analysis indicated that motor functional recovery in the TP group was superior to that in the NC group, and similar to that in the autograft (Auto) group. LT-NCLCs promoted axonal regrowth and remyelination by Schwann cells. Transplantation of LT-NCLCs is a promising approach for nerve regeneration treatment of massive peripheral nerve defects.

Original languageEnglish
Article number10071
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Induced Pluripotent Stem Cells
Nerve Regeneration
Neural Crest
Peripheral Nerves
Stem Cells
Transplantation
Cell Transplantation
Control Groups
Nerve Growth Factor Receptor
Schwann Cells
Autografts
Silicones
Sciatic Nerve
Thymocytes
Therapeutics
Antigens

ASJC Scopus subject areas

  • General

Cite this

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title = "Stem cells purified from human induced pluripotent stem cell-derived neural crest-like cells promote peripheral nerve regeneration",
abstract = "Strategies for therapeutic cell transplantation have been assessed for use in the treatment of massive peripheral nerve defects. To support safe and efficient cell transplantation, we have focused on the purification of cells using cell surface markers. Our group previously reported low-Affinity nerve growth factor receptor (LNGFR)-and thymocyte antigen-1 (THY-1)-positive neural crest-like cells (LT-NCLCs), generated from human induced pluripotent stem cells (hiPSCs). In the present study, we investigated the efficacy of transplantation of hiPSC-derived LT-NCLCs in a murine massive peripheral nerve defect model. Animals with a sciatic nerve defect were treated with a bridging silicone tube prefilled with LT-NCLCs or medium in the transplantation (TP) and negative control (NC) groups, respectively. The grafted LT-NCLCs survived and enhanced myelination and angiogenesis, as compared to the NC group. Behavioral analysis indicated that motor functional recovery in the TP group was superior to that in the NC group, and similar to that in the autograft (Auto) group. LT-NCLCs promoted axonal regrowth and remyelination by Schwann cells. Transplantation of LT-NCLCs is a promising approach for nerve regeneration treatment of massive peripheral nerve defects.",
author = "Hiroo Kimura and Takehito Ouchi and Shinsuke Shibata and Tsuyoshi Amemiya and Narihito Nagoshi and Taneaki Nakagawa and Morio Matsumoto and Hideyuki Okano and Masaya Nakamura and Kazuki Sato",
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AU - Kimura, Hiroo

AU - Ouchi, Takehito

AU - Shibata, Shinsuke

AU - Amemiya, Tsuyoshi

AU - Nagoshi, Narihito

AU - Nakagawa, Taneaki

AU - Matsumoto, Morio

AU - Okano, Hideyuki

AU - Nakamura, Masaya

AU - Sato, Kazuki

PY - 2018/12/1

Y1 - 2018/12/1

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