Stereochemistry of hydrogen addition to C-25 of desmosterol by sterol- Δ24-reductase of rat liver homogenate

T. Yagi, Noriko Kobayashi, M. Morisaki, N. Hara, Y. Fujimoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. 13C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.

Original languageEnglish
Pages (from-to)680-682
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume42
Issue number3
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Desmosterol
Stereochemistry
Sterols
Liver
Rats
Hydrogen
Oxidoreductases
Cholesterol
Nuclear magnetic resonance
Carbon-13 Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Stereochemistry of hydrogen addition to C-25 of desmosterol by sterol- Δ24-reductase of rat liver homogenate. / Yagi, T.; Kobayashi, Noriko; Morisaki, M.; Hara, N.; Fujimoto, Y.

In: Chemical and Pharmaceutical Bulletin, Vol. 42, No. 3, 1994, p. 680-682.

Research output: Contribution to journalArticle

@article{aa59a3bd44f643dc81da353f47f82a26,
title = "Stereochemistry of hydrogen addition to C-25 of desmosterol by sterol- Δ24-reductase of rat liver homogenate",
abstract = "Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. 13C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.",
author = "T. Yagi and Noriko Kobayashi and M. Morisaki and N. Hara and Y. Fujimoto",
year = "1994",
language = "English",
volume = "42",
pages = "680--682",
journal = "Chemical and Pharmaceutical Bulletin",
issn = "0009-2363",
publisher = "Pharmaceutical Society of Japan",
number = "3",

}

TY - JOUR

T1 - Stereochemistry of hydrogen addition to C-25 of desmosterol by sterol- Δ24-reductase of rat liver homogenate

AU - Yagi, T.

AU - Kobayashi, Noriko

AU - Morisaki, M.

AU - Hara, N.

AU - Fujimoto, Y.

PY - 1994

Y1 - 1994

N2 - Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. 13C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.

AB - Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. 13C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.

UR - http://www.scopus.com/inward/record.url?scp=0028291745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028291745&partnerID=8YFLogxK

M3 - Article

C2 - 8004717

AN - SCOPUS:0028291745

VL - 42

SP - 680

EP - 682

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

SN - 0009-2363

IS - 3

ER -