Stereochemistry of reduction of the C-24,25 double bond in the conversion of desmosterol into cholesterol

Kyoko Takahashi, Kenichiro Hashimoto, Ayako Fujiyama, Junko Yamada, Noriko Kobayashi, Masuo Morisaki, Sayaka Nakano, Noriyuki Hara, Yoshinori Fujimoto

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6 Citations (Scopus)


Feeding of the chemically prepared [24-13C, 24-2H]desmosterol to cell-free systems derived from rat liver and silkworm gut and to cultured cells of Oryza sativa followed by deuterium-decoupled 1H, 13C shift correlation NMR analysis of the biosynthesized cholesterol revealed the stereospecific incorporation of hydrogen atoms from the re-face of the C-24 position of desmosterol.

Original languageEnglish
Pages (from-to)341-344
Number of pages4
JournalTetrahedron Letters
Issue number2
Publication statusPublished - 2003 Jan 6



  • 3β-hydroxysterol Δ-reductase
  • Cholesterol biosynthesis
  • Desmosterol
  • Stereochemistry
  • Steroids and sterols

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Drug Discovery

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