Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector

Hideko Kanazawa; Yuko Kunito; Yoshikazu Matsushima; Shigeo Okubo; Fumiko Mashige

doi:10.1016/S0021-9673(99)01244-3

Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector

Hideko Kanazawa, Yuko Kunito, Yoshikazu Matsushima, Shigeo Okubo, Fumiko Mashige

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The chiral separation of lorazepam was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of lorazepam enantiomers was obtained on the column of β-cyclodextrin derivative immobilized silica gel under reversed-phase conditions. The enantiomeric separation and identification of lorazepam were succeeded by CD detector. The method described allows the quantitation of the stereoisomers of lorazepam in human plasma following the administration of a therapeutic dose of the racemic drug. Chiroptical detection is useful for the pharmacokinetic study of chiral drugs. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	181-188
Number of pages	8
Journal	Journal of Chromatography A
Volume	871
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(99)01244-3
Publication status	Published - 2000 Feb 25

Keywords

Benzodiazepines
Circular dichroism detection
Detection, LC
Enantiomer separation
Lorazepam

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(99)01244-3

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abstract = "The chiral separation of lorazepam was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of lorazepam enantiomers was obtained on the column of β-cyclodextrin derivative immobilized silica gel under reversed-phase conditions. The enantiomeric separation and identification of lorazepam were succeeded by CD detector. The method described allows the quantitation of the stereoisomers of lorazepam in human plasma following the administration of a therapeutic dose of the racemic drug. Chiroptical detection is useful for the pharmacokinetic study of chiral drugs. (C) 2000 Elsevier Science B.V.",

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T1 - Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector

AU - Kanazawa, Hideko

AU - Kunito, Yuko

AU - Matsushima, Yoshikazu

AU - Okubo, Shigeo

AU - Mashige, Fumiko

PY - 2000/2/25

Y1 - 2000/2/25

N2 - The chiral separation of lorazepam was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of lorazepam enantiomers was obtained on the column of β-cyclodextrin derivative immobilized silica gel under reversed-phase conditions. The enantiomeric separation and identification of lorazepam were succeeded by CD detector. The method described allows the quantitation of the stereoisomers of lorazepam in human plasma following the administration of a therapeutic dose of the racemic drug. Chiroptical detection is useful for the pharmacokinetic study of chiral drugs. (C) 2000 Elsevier Science B.V.

AB - The chiral separation of lorazepam was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of lorazepam enantiomers was obtained on the column of β-cyclodextrin derivative immobilized silica gel under reversed-phase conditions. The enantiomeric separation and identification of lorazepam were succeeded by CD detector. The method described allows the quantitation of the stereoisomers of lorazepam in human plasma following the administration of a therapeutic dose of the racemic drug. Chiroptical detection is useful for the pharmacokinetic study of chiral drugs. (C) 2000 Elsevier Science B.V.

KW - Benzodiazepines

KW - Circular dichroism detection

KW - Detection, LC

KW - Enantiomer separation

KW - Lorazepam

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ER -

Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector

Abstract

Keywords

ASJC Scopus subject areas

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