Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector

Hideko Kanazawa, Yuko Kunito, Yoshikazu Matsushima, Shigeo Okubo, Fumiko Mashige

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The chiral separation of lorazepam was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of lorazepam enantiomers was obtained on the column of β-cyclodextrin derivative immobilized silica gel under reversed-phase conditions. The enantiomeric separation and identification of lorazepam were succeeded by CD detector. The method described allows the quantitation of the stereoisomers of lorazepam in human plasma following the administration of a therapeutic dose of the racemic drug. Chiroptical detection is useful for the pharmacokinetic study of chiral drugs. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalJournal of Chromatography A
Volume871
Issue number1-2
DOIs
Publication statusPublished - 2000 Feb 25

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Lorazepam
Column chromatography
Circular Dichroism
Chromatography
Detectors
Plasmas
Plasma (human)
Stereoisomerism
Pharmacokinetics
Enantiomers
Silica Gel
Cyclodextrins
Pharmaceutical Preparations
Therapeutics

Keywords

  • Benzodiazepines
  • Circular dichroism detection
  • Detection, LC
  • Enantiomer separation
  • Lorazepam

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector. / Kanazawa, Hideko; Kunito, Yuko; Matsushima, Yoshikazu; Okubo, Shigeo; Mashige, Fumiko.

In: Journal of Chromatography A, Vol. 871, No. 1-2, 25.02.2000, p. 181-188.

Research output: Contribution to journalArticle

Kanazawa, Hideko ; Kunito, Yuko ; Matsushima, Yoshikazu ; Okubo, Shigeo ; Mashige, Fumiko. / Stereospecific analysis of lorazepam in plasma by chiral column chromatography with a circular dichroism-based detector. In: Journal of Chromatography A. 2000 ; Vol. 871, No. 1-2. pp. 181-188.
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