Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector

Hideko Kanazawa, Akane Tsubayashi, Yoshiko Nagata, Yoshikazu Matsushima, Chiharu Mori, Junko Kizu, Megumu Higaki

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250×4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalJournal of Chromatography A
Volume948
Issue number1-2
DOIs
Publication statusPublished - 2002 Mar 1

Keywords

  • Circular dichroism detection
  • Detection, LC
  • Enantiomer separation
  • Loxoprofens
  • Nonsteriodal antiinflammatory drugs
  • Profens

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector'. Together they form a unique fingerprint.

  • Cite this