Stimulation of antiviral activities of interferon by a liver extract preparation

Shoji Yokochi, Yoshiro Ishiwata, Hidetsugu Saito, Hirotoshi Ebinuma, Masaharu Tsuchiya, Hiromasa Ishii

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3 Citations (Scopus)

Abstract

The liver extract preparation Adelavin®9 (referred to as EXT-FAD hereinafter) is a combination product that consists of hog liver extract and flavin-adenine dinucleotide (FAD). EXT-FAD has been reported to improve the histology of chronic liver injury and is widely used in Japan. Interferon (IFN) has been used to treat viral hepatitis. In this study, the action of EXT-FAD on the antiviral activity of IFN was examined using experimental models of viral infection in vitro and in vivo. EXT-FAD significantly enhanced the 2',5'-oligoadenylate synthetase (2-5AS) activity in human peripheral blood mononuclear cells (PBMC) treated with human IFN-α-2a. EXT-FAD is likely to affect the late phase of IFN action to produce the antiviral activity. Combination of IFN-α-2a with EXT-FAD produced a synergistic inhibitory effect against herpes simplex virus type-1 (HSV-1) in vitro. The effect of EXT-FAD in combination with IFN-α-2a against influenza virus type A (Fluv A) was also examined and a similar effect to that found in the anti-HSV-1 assay was observed. In addition, administration of EXT-FAD to mice infected with HSV-1 significantly prolonged the mean survival periods. The intracellular redox state plays a role in inhibiting virus replication. EXT-FAD was found to increase the intracellular glutathione (GSH) level. EXT-FAD also enhanced the nitric oxide synthase (NOS) activity, which plays a role in antiviral effects. This result indicates that the effect of EXT-FAD on the antiviral activity of IFN is partially related to increased intracellular GSH levels and NOS activation.

Original languageEnglish
Pages (from-to)968-974
Number of pages7
JournalArzneimittel-Forschung/Drug Research
Volume47
Issue number8
Publication statusPublished - 1997 Sep 6

Keywords

  • Adelavin®
  • Antiviral effect
  • Flavin-adenine dinucleotide
  • Glutathione
  • Interferon
  • Liver extract preparation
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Drug Discovery

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  • Cite this

    Yokochi, S., Ishiwata, Y., Saito, H., Ebinuma, H., Tsuchiya, M., & Ishii, H. (1997). Stimulation of antiviral activities of interferon by a liver extract preparation. Arzneimittel-Forschung/Drug Research, 47(8), 968-974.