Stimulation of myelin basic protein gene transcription by fyn tyrosine kinase for myelination

Hisashi Umemori, Yasunori Kadowaki, Kazushige Hirosawa, Yutaka Yoshida, Katsunori Hironaka, Hideyuki Okano, Tadashi Yamamoto

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Myelin is synthesized about the time of birth. The Src-family tyrosine kinase Fyn is involved in the initial events of myelination. Fyn is present in myelin-forming cells and is activated through stimulation of celt surface receptors such as large myelin-associated glycoprotein (L-MAG). Here we show that Fyn stimulates transcription of the myelin basic protein (MBP) gene for myelination. MBP is a major component of the myelin membrane. In 4-week-old Fyn-deficient mice, MBP is significantly reduced, and electron microscopic analysis showed that myelination is delayed, compared with wild-type mice. The Fyn-deficient mice had thinner, more irregular myelin than the wild- type. We found that Fyn stimulates the promoter activity of the MBP gene by approximately sevenfold. The region responsible for the transactivation by Fyn is located between nucleotides -675 and -647 with respect to the transcription start site. Proteins binding to this region were found by gel shift study, and the binding activity correlates with Fyn activity during myelination. These results suggest that transactivation of the MBP gene by Fyn is important for myelination.

Original languageEnglish
Pages (from-to)1393-1397
Number of pages5
JournalJournal of Neuroscience
Volume19
Issue number4
DOIs
Publication statusPublished - 1999 Feb 15

Keywords

  • Developmental regulation
  • Fyn tyrosine kinase
  • Knock-out mouse
  • Myelin basic protein
  • Myelination
  • Transactivation

ASJC Scopus subject areas

  • Neuroscience(all)

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