Stimulation of neurotrophic factor secretion from 1321N1 human astrocytoma cells by novel diterpenoids, scabronines A and G

Yutaro Obara, Norimichi Nakahata, Takako Kita, Yoshiaki Takaya, Hironori Kobayashi, Shinzo Hosoi, Fumiyuki Kiuchi, Tomihisa Ohta, Yoshiteru Oshima, Yasushi Ohizumi

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Glial cells release neurotrophic factors that maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated with the conditioned medium of human astrocytoma cells (1321N1) incubated with the new diterpenoids, scabronines A and G, isolated from Sarcodon scabrosus, they changed their morphology and there was neurite outgrowth. The scabronines increased the expression of mRNA for nerve growth factor (NGF), and the secretion of NGF from 1321N1 cells in a concentration-dependent manner. However, the enhanced neurite outgrowth produced by the conditioned media was slightly inhibited by NGF neutralizing antibody, and the concentration of NGF released in response to the scabronines was insufficient to cause differentiation. These results suggest that scabronines cause the secretion of other factors together with NGF from 1321N1 cells. The diterpenoids are useful drugs to clarify the mechanism of synthesis and secretion of neurotrophic factors. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalEuropean journal of pharmacology
Volume370
Issue number1
DOIs
Publication statusPublished - 1999 Apr 1
Externally publishedYes

Keywords

  • 1321N1 human astrocytoma cell
  • NGF (nerve growth factor)
  • Neurite outgrowth
  • Neurotrophic factor
  • PC-12 cell
  • Scabronine

ASJC Scopus subject areas

  • Pharmacology

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