Stimulation of osteogenesis in bone defects implanted with biodegradable hydroxyapatite composed of rod-shaped particles under mechanical unloading

Tohru Ikeda, Yoshinori Gonda, Eri Tatsukawa, Yasuaki Shibata, Masanobu Kamitakahara, Takatoshi Okuda, Ikuho Yonezawa, Hisashi Kurosawa, Koji Ioku

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of thisSstudy was totevaluatertheninfluence of mechanical unloading on the repair of bone defects with implantation of biodegradable bone substitutes. Spherical granules of biodegradable hydroxyapatite composed of rod-shaped particles (RHA) or beta-tricalcium phosphate composed of rod-shaped particles (RTCP) were implanted into a bone defect created in the distal end of the right femur of 8-week-old Wistar rats. Two, 6, 10, and 22 weeks after implantation, part of the sciatic nerve in the thigh was resected and exposed to mechanical unloading for 2 weeks. Then, 4, 8, 12 and 24 weeks after implantation, repair of the bone defect was analyzed. As a control, the bone defect without implantation of ceramic granules was also analyzed. Both RHA and RTCP tended to be reduced, but the reduction was not obvious during the experimental period. At 12 and 24 weeks after implantation, the amount of newly formed bone in the animal implanted with RHA was significantly greater than that at 4 weeks after implantation, but that in the animal implanted with RTCP or without implantation was not significantly different. The number of osteoclasts in the region implanted with RHA was significantly larger than that of the region implanted with RTCP or without implantation at 12 and 24 weeks. The activities of alkaline phosphatase in osteoblasts and tartrate-resistant acid phosphatase in osteoclasts were remarkably increased in the bone defects with implantation compared with those in the bone defects without implantation. These results suggested that RHA stimulated osteogenesis and osteoclastogenesis even after 2 weeks of mechanical unloading, and that RHA could be expected to improve the repair of bone defects in patients under the condition of skeletal unloading.

Original languageEnglish
Pages (from-to)283-292
Number of pages10
JournalActa Histochemica et Cytochemica
Volume45
Issue number5
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Durapatite
Unloading
Osteogenesis
Bone
Bone and Bones
Defects
Repair
Osteoclasts
Animals
Hindlimb Suspension
Bone Substitutes
Osteoblasts
Ceramics
Sciatic Nerve
Acid Phosphatase
Thigh
Femur
Alkaline Phosphatase
Wistar Rats
Rats

Keywords

  • Beta-tricalcium phosphate
  • Bone metabolism
  • Bone substitute
  • Hydroxyapatite
  • Mechanical unloading

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Stimulation of osteogenesis in bone defects implanted with biodegradable hydroxyapatite composed of rod-shaped particles under mechanical unloading. / Ikeda, Tohru; Gonda, Yoshinori; Tatsukawa, Eri; Shibata, Yasuaki; Kamitakahara, Masanobu; Okuda, Takatoshi; Yonezawa, Ikuho; Kurosawa, Hisashi; Ioku, Koji.

In: Acta Histochemica et Cytochemica, Vol. 45, No. 5, 2012, p. 283-292.

Research output: Contribution to journalArticle

Ikeda, Tohru ; Gonda, Yoshinori ; Tatsukawa, Eri ; Shibata, Yasuaki ; Kamitakahara, Masanobu ; Okuda, Takatoshi ; Yonezawa, Ikuho ; Kurosawa, Hisashi ; Ioku, Koji. / Stimulation of osteogenesis in bone defects implanted with biodegradable hydroxyapatite composed of rod-shaped particles under mechanical unloading. In: Acta Histochemica et Cytochemica. 2012 ; Vol. 45, No. 5. pp. 283-292.
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AU - Gonda, Yoshinori

AU - Tatsukawa, Eri

AU - Shibata, Yasuaki

AU - Kamitakahara, Masanobu

AU - Okuda, Takatoshi

AU - Yonezawa, Ikuho

AU - Kurosawa, Hisashi

AU - Ioku, Koji

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AB - The aim of thisSstudy was totevaluatertheninfluence of mechanical unloading on the repair of bone defects with implantation of biodegradable bone substitutes. Spherical granules of biodegradable hydroxyapatite composed of rod-shaped particles (RHA) or beta-tricalcium phosphate composed of rod-shaped particles (RTCP) were implanted into a bone defect created in the distal end of the right femur of 8-week-old Wistar rats. Two, 6, 10, and 22 weeks after implantation, part of the sciatic nerve in the thigh was resected and exposed to mechanical unloading for 2 weeks. Then, 4, 8, 12 and 24 weeks after implantation, repair of the bone defect was analyzed. As a control, the bone defect without implantation of ceramic granules was also analyzed. Both RHA and RTCP tended to be reduced, but the reduction was not obvious during the experimental period. At 12 and 24 weeks after implantation, the amount of newly formed bone in the animal implanted with RHA was significantly greater than that at 4 weeks after implantation, but that in the animal implanted with RTCP or without implantation was not significantly different. The number of osteoclasts in the region implanted with RHA was significantly larger than that of the region implanted with RTCP or without implantation at 12 and 24 weeks. The activities of alkaline phosphatase in osteoblasts and tartrate-resistant acid phosphatase in osteoclasts were remarkably increased in the bone defects with implantation compared with those in the bone defects without implantation. These results suggested that RHA stimulated osteogenesis and osteoclastogenesis even after 2 weeks of mechanical unloading, and that RHA could be expected to improve the repair of bone defects in patients under the condition of skeletal unloading.

KW - Beta-tricalcium phosphate

KW - Bone metabolism

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KW - Hydroxyapatite

KW - Mechanical unloading

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