Stimulation of steroidogenic acute regulatory protein (StAR) gene expression by D-aspartate in rat Leydig cells

Yoshiko Nagata; Hiroshi Homma; Megumi Matsumoto; Kazuhiro Imai

doi:10.1016/S0014-5793(99)00840-6

Stimulation of steroidogenic acute regulatory protein (StAR) gene expression by D-aspartate in rat Leydig cells

Yoshiko Nagata, Hiroshi Homma, Megumi Matsumoto, Kazuhiro Imai

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

D-aspartate and human chorionic gonadotropin act synergistically to increase testosterone production in purified rat Leydig cells, and D-aspartate stimulates testosterone synthesis even in the absence of human chorionic gonadotropin stimulation. In addition, D-aspartate enhances steady-state cellular mRNA and protein levels of steroidogenic acute regulatory protein, which is a key regulatory factor in gonadal and adrenal steroidogenesis. D-aspartate therefore appears to increase testosterone production in rat Leydig cells by stimulating steroidogenic acute regulatory protein gene expression. To our knowledge, this is the first report demonstrating a direct effect of D-aspartate on gene expression in mammalian cells. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	317-320
Number of pages	4
Journal	FEBS Letters
Volume	454
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00840-6
Publication status	Published - 1999 Jul 9
Externally published	Yes

Keywords

D-aspartate
Leydig cell
StAR
Steroidogenic acute regulatory protein
Testosterone

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Stimulation of steroidogenic acute regulatory protein (StAR) gene expression by D-aspartate in rat Leydig cells",

abstract = "D-aspartate and human chorionic gonadotropin act synergistically to increase testosterone production in purified rat Leydig cells, and D-aspartate stimulates testosterone synthesis even in the absence of human chorionic gonadotropin stimulation. In addition, D-aspartate enhances steady-state cellular mRNA and protein levels of steroidogenic acute regulatory protein, which is a key regulatory factor in gonadal and adrenal steroidogenesis. D-aspartate therefore appears to increase testosterone production in rat Leydig cells by stimulating steroidogenic acute regulatory protein gene expression. To our knowledge, this is the first report demonstrating a direct effect of D-aspartate on gene expression in mammalian cells. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "D-aspartate, Leydig cell, StAR, Steroidogenic acute regulatory protein, Testosterone",

author = "Yoshiko Nagata and Hiroshi Homma and Megumi Matsumoto and Kazuhiro Imai",

note = "Funding Information: Acknowledgements: The authors express their sincere appreciation to Prof. D.M. Stocco (Texas Tech. University Health Sciences Center, Texas, USA) for his generous gift of anti-StAR antiserum. This study was supported in part by Grants-in-Aid for Scientific Research 11672163 from the Ministry of Education, Science, Sports and Culture of Japan.",

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doi = "10.1016/S0014-5793(99)00840-6",

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AU - Nagata, Yoshiko

AU - Homma, Hiroshi

AU - Matsumoto, Megumi

AU - Imai, Kazuhiro

N1 - Funding Information: Acknowledgements: The authors express their sincere appreciation to Prof. D.M. Stocco (Texas Tech. University Health Sciences Center, Texas, USA) for his generous gift of anti-StAR antiserum. This study was supported in part by Grants-in-Aid for Scientific Research 11672163 from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 1999/7/9

Y1 - 1999/7/9

N2 - D-aspartate and human chorionic gonadotropin act synergistically to increase testosterone production in purified rat Leydig cells, and D-aspartate stimulates testosterone synthesis even in the absence of human chorionic gonadotropin stimulation. In addition, D-aspartate enhances steady-state cellular mRNA and protein levels of steroidogenic acute regulatory protein, which is a key regulatory factor in gonadal and adrenal steroidogenesis. D-aspartate therefore appears to increase testosterone production in rat Leydig cells by stimulating steroidogenic acute regulatory protein gene expression. To our knowledge, this is the first report demonstrating a direct effect of D-aspartate on gene expression in mammalian cells. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - D-aspartate and human chorionic gonadotropin act synergistically to increase testosterone production in purified rat Leydig cells, and D-aspartate stimulates testosterone synthesis even in the absence of human chorionic gonadotropin stimulation. In addition, D-aspartate enhances steady-state cellular mRNA and protein levels of steroidogenic acute regulatory protein, which is a key regulatory factor in gonadal and adrenal steroidogenesis. D-aspartate therefore appears to increase testosterone production in rat Leydig cells by stimulating steroidogenic acute regulatory protein gene expression. To our knowledge, this is the first report demonstrating a direct effect of D-aspartate on gene expression in mammalian cells. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - D-aspartate

KW - Leydig cell

KW - StAR

KW - Steroidogenic acute regulatory protein

KW - Testosterone

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ER -

Stimulation of steroidogenic acute regulatory protein (StAR) gene expression by D-aspartate in rat Leydig cells

Abstract

Keywords

ASJC Scopus subject areas

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