Stimulation of the extrusion of protons and H+-ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaCl stress and its relation to external levels of Ca2+ ions

Yoshiyuki Nakamura, Kunihiro Kasamo, Makato Sakata, Eiji Ohta

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Abstract

Extrusion of protons as a response to high-NaCl stress in intact mung bean roots was investigated at different external concentrations of Ca2+ ions ([Ca2+]ex). The extrusion of protons was gradually enhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]ex diminished this enhancement of the extrusion. Vesicles of plasmalemma and tonoplast were prepared from the roots and the H+-translocating ATPase (H+-ATPase) activities associated with the two types of membrane and the H+-pyrophosphatase (H+-PPase) activity of the tonoplast were assayed. The plasmalemma ATPase was stimulated in parallel with dramatic increases in the intracellular concentration of Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]in and diminished the stimulation of ATPase activity. The tonoplast ATPase showed a rapid response to salt stress and was similarly stimulated even at high [Ca2+]M. The activities of both ATPases were, however, insensitive to concentrations of Na+ ions up to 100 HIM. By contrast, H+-PPase activity of the tonoplast was severely inhibited with increasing [Na+]in under salt stress and recovered with high [Ca2+]ex. These findings suggest that high-NaCl stress increases the intracellular concentration of Na+ ions in mung bean roots, which inhibits the tonoplast H+-PPase, and the activity of the plasmalemma H+-ATPase is thereby stimulated and regulates the cytoplasmic pH.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalPlant and Cell Physiology
Volume33
Issue number2
Publication statusPublished - 1992 Mar

Fingerprint

Pyrophosphatases
Tonoplast
Mung Bean
pyrophosphatases
Proton-Translocating ATPases
Extrusion
tonoplast
ATP Synthase
Inorganic Pyrophosphatase
mung beans
extrusion
protons
adenosinetriphosphatase
Adenosine Triphosphatases
Protons
Roots
Ions
ions
calcium
ion

Keywords

  • Ca2+
  • Extrusion of protons
  • H+-ATPase
  • H+-pyrophophatase
  • Mung beanVigna mungo root
  • Salt stress

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

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title = "Stimulation of the extrusion of protons and H+-ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaCl stress and its relation to external levels of Ca2+ ions",
abstract = "Extrusion of protons as a response to high-NaCl stress in intact mung bean roots was investigated at different external concentrations of Ca2+ ions ([Ca2+]ex). The extrusion of protons was gradually enhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]ex diminished this enhancement of the extrusion. Vesicles of plasmalemma and tonoplast were prepared from the roots and the H+-translocating ATPase (H+-ATPase) activities associated with the two types of membrane and the H+-pyrophosphatase (H+-PPase) activity of the tonoplast were assayed. The plasmalemma ATPase was stimulated in parallel with dramatic increases in the intracellular concentration of Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]in and diminished the stimulation of ATPase activity. The tonoplast ATPase showed a rapid response to salt stress and was similarly stimulated even at high [Ca2+]M. The activities of both ATPases were, however, insensitive to concentrations of Na+ ions up to 100 HIM. By contrast, H+-PPase activity of the tonoplast was severely inhibited with increasing [Na+]in under salt stress and recovered with high [Ca2+]ex. These findings suggest that high-NaCl stress increases the intracellular concentration of Na+ ions in mung bean roots, which inhibits the tonoplast H+-PPase, and the activity of the plasmalemma H+-ATPase is thereby stimulated and regulates the cytoplasmic pH.",
keywords = "Ca2+, Extrusion of protons, H+-ATPase, H+-pyrophophatase, Mung beanVigna mungo root, Salt stress",
author = "Yoshiyuki Nakamura and Kunihiro Kasamo and Makato Sakata and Eiji Ohta",
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T1 - Stimulation of the extrusion of protons and H+-ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaCl stress and its relation to external levels of Ca2+ ions

AU - Nakamura, Yoshiyuki

AU - Kasamo, Kunihiro

AU - Sakata, Makato

AU - Ohta, Eiji

PY - 1992/3

Y1 - 1992/3

N2 - Extrusion of protons as a response to high-NaCl stress in intact mung bean roots was investigated at different external concentrations of Ca2+ ions ([Ca2+]ex). The extrusion of protons was gradually enhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]ex diminished this enhancement of the extrusion. Vesicles of plasmalemma and tonoplast were prepared from the roots and the H+-translocating ATPase (H+-ATPase) activities associated with the two types of membrane and the H+-pyrophosphatase (H+-PPase) activity of the tonoplast were assayed. The plasmalemma ATPase was stimulated in parallel with dramatic increases in the intracellular concentration of Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]in and diminished the stimulation of ATPase activity. The tonoplast ATPase showed a rapid response to salt stress and was similarly stimulated even at high [Ca2+]M. The activities of both ATPases were, however, insensitive to concentrations of Na+ ions up to 100 HIM. By contrast, H+-PPase activity of the tonoplast was severely inhibited with increasing [Na+]in under salt stress and recovered with high [Ca2+]ex. These findings suggest that high-NaCl stress increases the intracellular concentration of Na+ ions in mung bean roots, which inhibits the tonoplast H+-PPase, and the activity of the plasmalemma H+-ATPase is thereby stimulated and regulates the cytoplasmic pH.

AB - Extrusion of protons as a response to high-NaCl stress in intact mung bean roots was investigated at different external concentrations of Ca2+ ions ([Ca2+]ex). The extrusion of protons was gradually enhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]ex diminished this enhancement of the extrusion. Vesicles of plasmalemma and tonoplast were prepared from the roots and the H+-translocating ATPase (H+-ATPase) activities associated with the two types of membrane and the H+-pyrophosphatase (H+-PPase) activity of the tonoplast were assayed. The plasmalemma ATPase was stimulated in parallel with dramatic increases in the intracellular concentration of Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]in and diminished the stimulation of ATPase activity. The tonoplast ATPase showed a rapid response to salt stress and was similarly stimulated even at high [Ca2+]M. The activities of both ATPases were, however, insensitive to concentrations of Na+ ions up to 100 HIM. By contrast, H+-PPase activity of the tonoplast was severely inhibited with increasing [Na+]in under salt stress and recovered with high [Ca2+]ex. These findings suggest that high-NaCl stress increases the intracellular concentration of Na+ ions in mung bean roots, which inhibits the tonoplast H+-PPase, and the activity of the plasmalemma H+-ATPase is thereby stimulated and regulates the cytoplasmic pH.

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