Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts

Kazuki Nabeshima, Jun ichi Kishi, Takao Kurogi, Naoto Komada, Hiroaki Kataoka, Yasunori Okada, Masashi Koono

Research output: Contribution to journalArticle

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Abstract

The co-cultures of five different human tumor cell lines with human normal fibroblasts significantly stimulated the production of tissue inhibitors of metalloproteinases-1 (TIMP-1) when compared to cultures of individual cells. In the co-culture of T24 human urinary bladder carcinoma cells and CCD18 human fibroblasts, production of both TIMP-1 and metalloproteinases was stimulated, and the stimulatory effects were dependent on the cellular ratio between the fibroblasts and carcinoma cells. On day 6 of culture, collagenase and stromelysin were stimulated at a ratio of CCD18 fibroblasts to T24 cells of 1:0.1, while the maximum TIMP-1 production occurred at a ratio of 1:1. Thus, the cellular ratio in the interaction of carcinoma cells with host fibroblasts affects the production of TIMP-1 and metalloproteinases and hence modulates the balance between them.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalCancer Letters
Volume78
Issue number1-3
DOIs
Publication statusPublished - 1994 Apr 1

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Tissue Inhibitor of Metalloproteinase-1
Metalloproteases
Coculture Techniques
Fibroblasts
Neoplasms
Carcinoma
Matrix Metalloproteinase 3
Collagenases
Tumor Cell Line
Cell Communication
Urinary Bladder
Cell Culture Techniques

Keywords

  • Cell-cell interaction
  • Matrix metalloproteinase
  • TIMP-1
  • Tumor invasion

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Nabeshima, K., Kishi, J. I., Kurogi, T., Komada, N., Kataoka, H., Okada, Y., & Koono, M. (1994). Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts. Cancer Letters, 78(1-3), 133-140. https://doi.org/10.1016/0304-3835(94)90042-6

Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts. / Nabeshima, Kazuki; Kishi, Jun ichi; Kurogi, Takao; Komada, Naoto; Kataoka, Hiroaki; Okada, Yasunori; Koono, Masashi.

In: Cancer Letters, Vol. 78, No. 1-3, 01.04.1994, p. 133-140.

Research output: Contribution to journalArticle

Nabeshima, K, Kishi, JI, Kurogi, T, Komada, N, Kataoka, H, Okada, Y & Koono, M 1994, 'Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts', Cancer Letters, vol. 78, no. 1-3, pp. 133-140. https://doi.org/10.1016/0304-3835(94)90042-6
Nabeshima, Kazuki ; Kishi, Jun ichi ; Kurogi, Takao ; Komada, Naoto ; Kataoka, Hiroaki ; Okada, Yasunori ; Koono, Masashi. / Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts. In: Cancer Letters. 1994 ; Vol. 78, No. 1-3. pp. 133-140.
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