Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts

Kazuki Nabeshima, Jun ichi Kishi, Takao Kurogi, Naoto Komada, Hiroaki Kataoka, Yasunori Okada, Masashi Koono

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The co-cultures of five different human tumor cell lines with human normal fibroblasts significantly stimulated the production of tissue inhibitors of metalloproteinases-1 (TIMP-1) when compared to cultures of individual cells. In the co-culture of T24 human urinary bladder carcinoma cells and CCD18 human fibroblasts, production of both TIMP-1 and metalloproteinases was stimulated, and the stimulatory effects were dependent on the cellular ratio between the fibroblasts and carcinoma cells. On day 6 of culture, collagenase and stromelysin were stimulated at a ratio of CCD18 fibroblasts to T24 cells of 1:0.1, while the maximum TIMP-1 production occurred at a ratio of 1:1. Thus, the cellular ratio in the interaction of carcinoma cells with host fibroblasts affects the production of TIMP-1 and metalloproteinases and hence modulates the balance between them.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalCancer Letters
Volume78
Issue number1-3
DOIs
Publication statusPublished - 1994 Apr 1

Keywords

  • Cell-cell interaction
  • Matrix metalloproteinase
  • TIMP-1
  • Tumor invasion

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Nabeshima, K., Kishi, J. I., Kurogi, T., Komada, N., Kataoka, H., Okada, Y., & Koono, M. (1994). Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts. Cancer Letters, 78(1-3), 133-140. https://doi.org/10.1016/0304-3835(94)90042-6