Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in mice

Tomoyuki Tanaka, Toshio Suda, Junko Suda, Tohru Inoue, Yoko Hirabayashi, Hisamaru Hirai, Fumimaro Takaku, Yasusada Miura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Granulocyte colony-stimulating factor (G-CSF) was reported to increase the number of colony-forming units-spleen (CFU-S) and multilineage colonies as well as myeloid-committed cells. We investigated the effects of G-CSF on myeloid progenitors and primitive stem cells in a mouse bone marrow transplantation (BMT) system. Lethally irradiated mice received BM cells from untreated or 5-fluorouracil - treated mice, and then were administered G-CSF or carrier buffer (control) for 5 days from immediately after BMT. A pre-CFU-S assay was performed by the repeated transplantation of BM cells from the first BMT recipients to other mice. By the method of polymerase chain reaction, most of the spleen colonies in the secondary recipients were confirmed to be derived from the first donors. G-CSF did not increase the peripheral white blood cell count significantly, but did increase the number of immature myeloid cells and granulocyte-macrophage colony-forming cells in the BM. The number of erythroid cells in the BM was initially suppressed and then increased by G-CSF treatment. In addition, the pre-CFU-S assay showed an increase in pre-CFU-S cells due to G-CSF administration. The number of spleen colonies of first BMT recipients did not increase, but a higher percentage of them were committed to a certain lineage by G-CSF treatment. These findings suggest that G-CSF has important roles in the early stages of hematopoiesis.

Original languageEnglish
Pages (from-to)2597-2602
Number of pages6
JournalBlood
Volume77
Issue number12
Publication statusPublished - 1991 Jun 15

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Granulocyte Colony-Stimulating Factor
Stem Cells
Spleen
Bone Marrow Transplantation
Bone
Colony-Forming Units Assay
Myeloid Cells
Assays
Erythroid Cells
Macrophages
Polymerase chain reaction
Hematopoiesis
Stem cells
Leukocyte Count
Granulocytes
Fluorouracil
Buffers
Blood
Transplantation
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Tanaka, T., Suda, T., Suda, J., Inoue, T., Hirabayashi, Y., Hirai, H., ... Miura, Y. (1991). Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in mice. Blood, 77(12), 2597-2602.

Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in mice. / Tanaka, Tomoyuki; Suda, Toshio; Suda, Junko; Inoue, Tohru; Hirabayashi, Yoko; Hirai, Hisamaru; Takaku, Fumimaro; Miura, Yasusada.

In: Blood, Vol. 77, No. 12, 15.06.1991, p. 2597-2602.

Research output: Contribution to journalArticle

Tanaka, T, Suda, T, Suda, J, Inoue, T, Hirabayashi, Y, Hirai, H, Takaku, F & Miura, Y 1991, 'Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in mice', Blood, vol. 77, no. 12, pp. 2597-2602.
Tanaka, Tomoyuki ; Suda, Toshio ; Suda, Junko ; Inoue, Tohru ; Hirabayashi, Yoko ; Hirai, Hisamaru ; Takaku, Fumimaro ; Miura, Yasusada. / Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in mice. In: Blood. 1991 ; Vol. 77, No. 12. pp. 2597-2602.
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abstract = "Granulocyte colony-stimulating factor (G-CSF) was reported to increase the number of colony-forming units-spleen (CFU-S) and multilineage colonies as well as myeloid-committed cells. We investigated the effects of G-CSF on myeloid progenitors and primitive stem cells in a mouse bone marrow transplantation (BMT) system. Lethally irradiated mice received BM cells from untreated or 5-fluorouracil - treated mice, and then were administered G-CSF or carrier buffer (control) for 5 days from immediately after BMT. A pre-CFU-S assay was performed by the repeated transplantation of BM cells from the first BMT recipients to other mice. By the method of polymerase chain reaction, most of the spleen colonies in the secondary recipients were confirmed to be derived from the first donors. G-CSF did not increase the peripheral white blood cell count significantly, but did increase the number of immature myeloid cells and granulocyte-macrophage colony-forming cells in the BM. The number of erythroid cells in the BM was initially suppressed and then increased by G-CSF treatment. In addition, the pre-CFU-S assay showed an increase in pre-CFU-S cells due to G-CSF administration. The number of spleen colonies of first BMT recipients did not increase, but a higher percentage of them were committed to a certain lineage by G-CSF treatment. These findings suggest that G-CSF has important roles in the early stages of hematopoiesis.",
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