Strain-specific phenotypes of airway inflammation and bronchial hyperresponsiveness induced by epicutaneous allergen sensitization in BALB/c and C57BL/6 mice

Motohiro Kodama, Koichiro Asano, Tsuyoshi Oguma, Shizuko Kagawa, Katsuyoshi Tomomatsu, Misa Wakaki, Takahisa Takihara, Soichiro Ueda, Nao Ohmori, Hiromi Ogura, Jun Miyata, Kyuto Tanaka, Nobufumi Kamiishi, Koichi Fukunaga, Koichi Sayama, Eiji Ikeda, Taku Miyasho, Akitoshi Ishizaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Allergen sensitization through a disrupted skin barrier appears to play a prominent role in the development of atopic diseases, including allergic asthma. The role of the genetic background in immunological and physiological phenotypes induced by epicutaneous sensitization is undetermined. Methods: BALB/c and C57BL/6 mice were sensitized either epicutaneously by patch application of ovalbumin (OVA) or systemically by intraperitoneal injection of OVA with alum before exposure to aerosolized OVA. The concentrations of OVA-specific immunoglobulin in serum and cytokines in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay. The severity of airway inflammation was evaluated by cell counts in BALF, and bronchial responsiveness to methacholine was measured by the flexiVent system. Results: The production of OVA-specific IgG1 and IgE was greater in the epicutaneously sensitized BALB/c than C57BL/6 mice. In contrast, both eosinophilic airway inflammation and bronchial responsiveness to methacholine were more prominent in the C57BL/6 than in the BALB/c mice. The concentrations of interleukin-4 increased significantly in the BALF from C57BL/6 mice only. No between-strain differences were observed after intraperitoneal sensitization. Conclusions: The C57BL/6 mouse is a more appropriate model than the BALB/c mouse to study the relationship between skin barrier dysfunction and the pathogenesis of allergic asthma.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume152
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2010 Jun

Fingerprint

Ovalbumin
Inbred C57BL Mouse
Allergens
Bronchoalveolar Lavage Fluid
Inflammation
Phenotype
Methacholine Chloride
Asthma
Skin
Intraperitoneal Injections
Interleukin-4
Immunoglobulin E
Immunoglobulins
Cell Count
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Cytokines
Serum

Keywords

  • Allergic airway inflammation
  • Asthma model
  • Bronchial responsiveness
  • C57BL/6
  • Epicutaneous sensitization
  • Skin barrier dysfunction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Strain-specific phenotypes of airway inflammation and bronchial hyperresponsiveness induced by epicutaneous allergen sensitization in BALB/c and C57BL/6 mice. / Kodama, Motohiro; Asano, Koichiro; Oguma, Tsuyoshi; Kagawa, Shizuko; Tomomatsu, Katsuyoshi; Wakaki, Misa; Takihara, Takahisa; Ueda, Soichiro; Ohmori, Nao; Ogura, Hiromi; Miyata, Jun; Tanaka, Kyuto; Kamiishi, Nobufumi; Fukunaga, Koichi; Sayama, Koichi; Ikeda, Eiji; Miyasho, Taku; Ishizaka, Akitoshi.

In: International Archives of Allergy and Immunology, Vol. 152, No. SUPPL. 1, 06.2010, p. 67-74.

Research output: Contribution to journalArticle

Kodama, M, Asano, K, Oguma, T, Kagawa, S, Tomomatsu, K, Wakaki, M, Takihara, T, Ueda, S, Ohmori, N, Ogura, H, Miyata, J, Tanaka, K, Kamiishi, N, Fukunaga, K, Sayama, K, Ikeda, E, Miyasho, T & Ishizaka, A 2010, 'Strain-specific phenotypes of airway inflammation and bronchial hyperresponsiveness induced by epicutaneous allergen sensitization in BALB/c and C57BL/6 mice', International Archives of Allergy and Immunology, vol. 152, no. SUPPL. 1, pp. 67-74. https://doi.org/10.1159/000312128
Kodama, Motohiro ; Asano, Koichiro ; Oguma, Tsuyoshi ; Kagawa, Shizuko ; Tomomatsu, Katsuyoshi ; Wakaki, Misa ; Takihara, Takahisa ; Ueda, Soichiro ; Ohmori, Nao ; Ogura, Hiromi ; Miyata, Jun ; Tanaka, Kyuto ; Kamiishi, Nobufumi ; Fukunaga, Koichi ; Sayama, Koichi ; Ikeda, Eiji ; Miyasho, Taku ; Ishizaka, Akitoshi. / Strain-specific phenotypes of airway inflammation and bronchial hyperresponsiveness induced by epicutaneous allergen sensitization in BALB/c and C57BL/6 mice. In: International Archives of Allergy and Immunology. 2010 ; Vol. 152, No. SUPPL. 1. pp. 67-74.
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abstract = "Background: Allergen sensitization through a disrupted skin barrier appears to play a prominent role in the development of atopic diseases, including allergic asthma. The role of the genetic background in immunological and physiological phenotypes induced by epicutaneous sensitization is undetermined. Methods: BALB/c and C57BL/6 mice were sensitized either epicutaneously by patch application of ovalbumin (OVA) or systemically by intraperitoneal injection of OVA with alum before exposure to aerosolized OVA. The concentrations of OVA-specific immunoglobulin in serum and cytokines in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay. The severity of airway inflammation was evaluated by cell counts in BALF, and bronchial responsiveness to methacholine was measured by the flexiVent system. Results: The production of OVA-specific IgG1 and IgE was greater in the epicutaneously sensitized BALB/c than C57BL/6 mice. In contrast, both eosinophilic airway inflammation and bronchial responsiveness to methacholine were more prominent in the C57BL/6 than in the BALB/c mice. The concentrations of interleukin-4 increased significantly in the BALF from C57BL/6 mice only. No between-strain differences were observed after intraperitoneal sensitization. Conclusions: The C57BL/6 mouse is a more appropriate model than the BALB/c mouse to study the relationship between skin barrier dysfunction and the pathogenesis of allergic asthma.",
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T1 - Strain-specific phenotypes of airway inflammation and bronchial hyperresponsiveness induced by epicutaneous allergen sensitization in BALB/c and C57BL/6 mice

AU - Kodama, Motohiro

AU - Asano, Koichiro

AU - Oguma, Tsuyoshi

AU - Kagawa, Shizuko

AU - Tomomatsu, Katsuyoshi

AU - Wakaki, Misa

AU - Takihara, Takahisa

AU - Ueda, Soichiro

AU - Ohmori, Nao

AU - Ogura, Hiromi

AU - Miyata, Jun

AU - Tanaka, Kyuto

AU - Kamiishi, Nobufumi

AU - Fukunaga, Koichi

AU - Sayama, Koichi

AU - Ikeda, Eiji

AU - Miyasho, Taku

AU - Ishizaka, Akitoshi

PY - 2010/6

Y1 - 2010/6

N2 - Background: Allergen sensitization through a disrupted skin barrier appears to play a prominent role in the development of atopic diseases, including allergic asthma. The role of the genetic background in immunological and physiological phenotypes induced by epicutaneous sensitization is undetermined. Methods: BALB/c and C57BL/6 mice were sensitized either epicutaneously by patch application of ovalbumin (OVA) or systemically by intraperitoneal injection of OVA with alum before exposure to aerosolized OVA. The concentrations of OVA-specific immunoglobulin in serum and cytokines in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay. The severity of airway inflammation was evaluated by cell counts in BALF, and bronchial responsiveness to methacholine was measured by the flexiVent system. Results: The production of OVA-specific IgG1 and IgE was greater in the epicutaneously sensitized BALB/c than C57BL/6 mice. In contrast, both eosinophilic airway inflammation and bronchial responsiveness to methacholine were more prominent in the C57BL/6 than in the BALB/c mice. The concentrations of interleukin-4 increased significantly in the BALF from C57BL/6 mice only. No between-strain differences were observed after intraperitoneal sensitization. Conclusions: The C57BL/6 mouse is a more appropriate model than the BALB/c mouse to study the relationship between skin barrier dysfunction and the pathogenesis of allergic asthma.

AB - Background: Allergen sensitization through a disrupted skin barrier appears to play a prominent role in the development of atopic diseases, including allergic asthma. The role of the genetic background in immunological and physiological phenotypes induced by epicutaneous sensitization is undetermined. Methods: BALB/c and C57BL/6 mice were sensitized either epicutaneously by patch application of ovalbumin (OVA) or systemically by intraperitoneal injection of OVA with alum before exposure to aerosolized OVA. The concentrations of OVA-specific immunoglobulin in serum and cytokines in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay. The severity of airway inflammation was evaluated by cell counts in BALF, and bronchial responsiveness to methacholine was measured by the flexiVent system. Results: The production of OVA-specific IgG1 and IgE was greater in the epicutaneously sensitized BALB/c than C57BL/6 mice. In contrast, both eosinophilic airway inflammation and bronchial responsiveness to methacholine were more prominent in the C57BL/6 than in the BALB/c mice. The concentrations of interleukin-4 increased significantly in the BALF from C57BL/6 mice only. No between-strain differences were observed after intraperitoneal sensitization. Conclusions: The C57BL/6 mouse is a more appropriate model than the BALB/c mouse to study the relationship between skin barrier dysfunction and the pathogenesis of allergic asthma.

KW - Allergic airway inflammation

KW - Asthma model

KW - Bronchial responsiveness

KW - C57BL/6

KW - Epicutaneous sensitization

KW - Skin barrier dysfunction

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