Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice

Kaori Taniwaki, Hiroshi Fukamachi, Kiyoshi Komori, Yohei Ohtake, Takahiro Nonaka, Takeharu Sakamoto, Takayuki Shiomi, Yasunori Okada, Takeshi Itoh, Shigeyoshi Itohara, Motoharu Seiki, Ikuo Yana

Research output: Contribution to journalArticle

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Abstract

Matrix metalloproteinase-2 (MMP-2) is a stroma-derived MMP belonging to the type IV collagenase family. It is believed to mediate tumor cell behavior by degrading deposits of type IV collagen, a major component of the basement membrane. The membrane type 1-MMP (MT1-MMP) is a highly potent activator of MMP-2 and is expressed in many tumor and stromal cells. However, the roles played by stromal MMP-2 in tumor progression in vivo remain poorly understood. We established a colon epithelial cell line from an Mt1-mmp-/- mouse strain and transfected these cells with an inducible expression system for MT1-MMP (MT1rev cells). Following s.c. implantation into Mmp-2+/+ mice and induction of MT1-MMP expression, MT1rev cells grew rapidly, whereas they grew very slowly in Mmp-2-/- mice, even in the presence of MT1-MMP. This MT1-MMP-dependent tumor growth of MT1rev cells was enhanced in Mmp-2-/- mice as long as MMP-2 was supplied via transfection or coimplantation of MMP-2-positive fibroblasts. MT1rev cells cultured in vitro in a three-dimensional collagen gel matrix also required the MT1-MMP/MMP-2 axis for rapid proliferation. MT1rev cells deposit type IV collagen primarily at the cell-collagen interface, and these deposits seem scarce at sites of invasion and proliferation. These data suggest that cooperation between stroma-derived MMP-2 and tumor-derived MT1-MMP may play a role in tumor invasion and proliferation via remodeling of the tumor-associated basement membrane. To our knowledge, this is the first study demonstrating that MT1-MMP-dependent tumor growth in vivo requires stromal-derived MMP-2. It also suggests that MMP-2 represents a potential target for tumor therapeutics.

Original languageEnglish
Pages (from-to)4311-4319
Number of pages9
JournalCancer Research
Volume67
Issue number9
DOIs
Publication statusPublished - 2007 May 1

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Matrix Metalloproteinase 14
Matrix Metalloproteinase 2
Matrix Metalloproteinases
Growth
Neoplasms
Membranes
Collagen Type IV
Basement Membrane
Collagen
Collagenases
Stromal Cells
Transfection
Cultured Cells
Colon
Fibroblasts
Gels
Epithelial Cells
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Taniwaki, K., Fukamachi, H., Komori, K., Ohtake, Y., Nonaka, T., Sakamoto, T., ... Yana, I. (2007). Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice. Cancer Research, 67(9), 4311-4319. https://doi.org/10.1158/0008-5472.CAN-06-4761

Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice. / Taniwaki, Kaori; Fukamachi, Hiroshi; Komori, Kiyoshi; Ohtake, Yohei; Nonaka, Takahiro; Sakamoto, Takeharu; Shiomi, Takayuki; Okada, Yasunori; Itoh, Takeshi; Itohara, Shigeyoshi; Seiki, Motoharu; Yana, Ikuo.

In: Cancer Research, Vol. 67, No. 9, 01.05.2007, p. 4311-4319.

Research output: Contribution to journalArticle

Taniwaki, K, Fukamachi, H, Komori, K, Ohtake, Y, Nonaka, T, Sakamoto, T, Shiomi, T, Okada, Y, Itoh, T, Itohara, S, Seiki, M & Yana, I 2007, 'Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice', Cancer Research, vol. 67, no. 9, pp. 4311-4319. https://doi.org/10.1158/0008-5472.CAN-06-4761
Taniwaki, Kaori ; Fukamachi, Hiroshi ; Komori, Kiyoshi ; Ohtake, Yohei ; Nonaka, Takahiro ; Sakamoto, Takeharu ; Shiomi, Takayuki ; Okada, Yasunori ; Itoh, Takeshi ; Itohara, Shigeyoshi ; Seiki, Motoharu ; Yana, Ikuo. / Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice. In: Cancer Research. 2007 ; Vol. 67, No. 9. pp. 4311-4319.
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